ELISA Kit DIY Materials for Fibroblast Growth Factor 15 (FGF15)

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Fibroblast Growth Factor 15 (FGF15). No significant cross-reactivity or interference between Fibroblast Growth Factor 15 (FGF15) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Endocrinology. Naringenin Prevents Obesity, Hepatic Steatosis, and Glucose Intolerance in Male Mice Independent of Fibroblast Growth Factor 21 PubMed: 25774553
Inflammatory Bowel Diseases Alterations in Enterohepatic Fgf15 Signaling and Changes in Bile Acid Composition Depend on Localization of Murine Intestinal Inflammation pubmed:27580383
J Lipid Res. Commensal bacteria at the crossroad between cholesterol homeostasis and chronic inflammation in atherosclerosis. pubmed:28130274
BMC Endocrine Disorders The relationship between bile acid concentration, glucagon-like-peptide 1, fibroblast growth factor 15 and bile acid receptors in rats during progression of glucose intolerance 10.1186/s12902-017-0211-5
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KSL154Ra01 ELISA Kit DIY Materials for Fibroblast Growth Factor 15 (FGF15) Main materials for "Do It(ELISA Kit) Yourself"