Lysis Buffer for Cells and Tissue
First Edition (Revised on April, 2016)
Lysis buffer is used to lyse cells under non-denaturing buffer conditions, it consists of surfactant, a protease inhibitor, etc. It’s mainly used for protein extraction from cells and tissues. The four lysis buffer are optimized from traditional lysis buffer. They’re suitable for lysis of cell membrane, cytoplasm and nucleus etc., which will help the release of proteins and do good for later extraction, ELISA, Western Blot, etc.
The product is one of the following:
In order to avoid protein degradation, it is recommended to add 1-2mM PMSF into the Lysis Buffer before usage.
|Lysis Buffer 1||Membrane proteins including surface proteins, receptors|
|Lysis Buffer 2||Cytoplasmic proteins, structural proteins, etc.|
|Lysis Buffer 3||Cytoplasmic proteins, nuclear matrix proteins, membrane proteins, etc.|
|Lysis Buffer 4||Nuclear matrix proteins, endosome and other organelles protein|
|REAGENT||Quantity||Usage and Dosage|
|Lysis Buffer||20ml(1×)||10 7 cells/50mg tissues by 1 ml lysis buffer.|
It's applicable on most experiments, such as SDS-PAGE, Western Blotting (WB), Immunoprecipitation (IP), Co-IP, ELISA etc.
STORAGE AND PERIOD OF VALIDITY
Store at 4°C for frequent use. Stored at -20°C for one year. Avoid repeated freeze-thaw cycles.
1.Please avoid freeze/thaw cycles, it’s suggested to aliquot when receiving.
2.The lysis process is suggested on the ice.
3.There might be salt precipitation in the lysis buffer after long-term storage at 4oC, but it will dissolve after it is placed at room temperature. So this phenomenon is normal, and it would not affect the result of the experiment.
4.Lysis buffer should be chosen according to the characteristics of samples and experimental purposes. The type of lysis buffer and optimal experimental condition should be determined by customers, and a pilot experiment is suggested.