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Recombinant Fibronectin Type III Domain Containing Protein 5 (FNDC5)

FRCP2; Irisin; Fibronectin type III repeat-containing protein 2

  • Product No.RPN576Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane
  • Predicted Molecular Mass27.4kDa
  • Accurate Molecular Mass34kDa(Analysis of differences refer to the manual)
  • Residues & TagsAsp29~Thr149 with Two N-terminal Tags, His-tag and SUMO-tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point5.2
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$   US$   US$   US$   US$  
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Diabetes Research and Clinical Practice Serum irisin levels in new-onset type 2 diabetes PubMed: 23369227
Journal of diabetes and its complications Lower circulating irisin is associated with type 2 diabetes mellitus Pubmed: 23619195
Horm Metab Res. Inconsistency in circulating irisin levels: what is really happening Pubmed:24459033
Int J Obes (Lond). Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice Pubmed:25199621
Molecular and Cellular Endocrinology Fenofibrate (PPARalpha agonist) induces beige cell formation in subcutaneous white adipose tissue from diet-induced male obese mice Pubmed:25576856
journal of diabetes Lower irisin level in patients with type 2 diabetes mellitus: A case-control study and meta-analysis PubMed: 25494632
The Journal of Maternal-Fetal & Neonatal Medicine Maternal circulating levels of irisin in intrahepatic cholestasis of pregnancy PubMed: 26689349
Hormone and Metabolic Research Lower Circulating Irisin Level in Patients with Diabetes Mellitus: A Systematic Review and Meta-Analysis Pubmed:27300472
Nature Reviews Endocrinology Physiology and role of irisin in glucose homeostasis. pubmed:28211512
Journal of Molecular and Cellular Cardiology Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193
Biochemical and Biophysical Research Communications Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma pubmed:28867187
PLoS One Differential actions of PPAR-α and PPAR-β/δ on beige adipocyte formation: A study in the subcutaneous white adipose tissue of obese male mice Pubmed:29351550
International Brazilian Journal of Urology The Diagnostic Value of FNDC5/Irisin in Renal Cell Cancer. Pubmed:29522296
Redox Biology Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative … Doi: 10.1016/j.redox.2018.10.019
Antioxidants & redox signaling Low serum irisin concentration is associated with poor outcomes in patients with acute pancreatitis and irisin administration protects against experimental acute … Pubmed: 31250660
Journal of Maternal-Fetal & Neonatal Medicine Maternal serum, placental, and umbilical venous blood irisin levels in intrahepatic cholestasis of pregnancy Pubmed: 31590596
Biomed Pharmacother Identification of irisin as a therapeutic agent that inhibits oxidative stress and fibrosis in a murine model of chronic pancreatitis Pubmed: 32199226
Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury Pubmed: 31976032
Serum Irisin Predicts Posthepatectomy Complications in Patients with Hepatocellular Carcinoma Pubmed: 31976024
DOMESTIC ANIMAL ENDOCRINOLOGY The myokine Irisin: localization and effects in swine late medium and large antral ovarian follicle Pubmed: 33120167
Journal of Biomaterials and Tissue Engineering The Combination of Aerobic and Resistance Exercise Induces Weight Loss via the PGC-1α/Irisin/UCP-1 Pathway
Evaluation of a recombinant bacillus calmette-guérin vaccine expressing P39-L7/L12 of Brucella melitensis: An immunization strategy against brucellosis in BALB/c …
Eur Rev Med Pharmacol Sci Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients 33755963
Metabolites Pattern of Adiponectin, Osteocalcin, Irisin, FGF-21, and MCP-1 According to the Body Size Phenotype: Could They Be Markers of Metabolic Health in Mexican-Mestizo … 34822430
Eat Weight Disord Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice 34370270
Ir J Med Sci Eight-week exercise program improved the quality of life of Alzheimer's patients through functional, cognitive, and biochemical parameters Pubmed:35604540
Biomed Pharmacother Irisin attenuates sepsis-induced cardiac dysfunction by attenuating inflammation-induced pyroptosis through a mitochondrial ubiquitin ligase-dependent … Pubmed:35653888
Journal of King Saud University - Science Interaction of nitric oxide synthase and mitochondrial ATP-sensitive potassium channels in protective impacts of combination therapy with irisin …
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPN576Ra01 Recombinant Fibronectin Type III Domain Containing Protein 5 (FNDC5) Positive Control; Immunogen; SDS-PAGE; WB.
SEN576Ra ELISA Kit for Fibronectin Type III Domain Containing Protein 5 (FNDC5) Enzyme-linked immunosorbent assay for Antigen Detection.
LMN576Ra Multiplex Assay Kit for Fibronectin Type III Domain Containing Protein 5 (FNDC5) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.