Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)
PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase
- Product No.RPH839Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationCytoplasm
- Predicted Molecular Mass30.6kDa
- Accurate Molecular Mass30kDa(Analysis of differences refer to the manual)
- Residues & TagsAla74~Ile333 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 97%
- Isoelectric Point7.2
-
Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 170
For more details, please contact local distributors! US$ 424 US$ 848 US$ 2544 US$ 6360
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
- Endotoxin Removal Kit
- BCA Protein Quantification Kit
- Protein Labeling Customized Service
- Molecular Mass Marker for Protein
- Recombinant Protein Customized Service
- Monoclonal Antibody Customized Service
- Polyclonal Antibody Customized Service
- Protein Activity Test Experiment Service
- Immunoprecipitation (IP) Experiment Service
- Buffer
- Endotoxin Removal Kit II
- Real Time PCR Experimental Service
- Spike RBD Protein (S-RBD)
- Protein G
- Protein A
Magazine | Citations |
Molecular & Cellular Proteomics | Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic Analysis Mcponline: 008821 |
Molecular & Cellular Proteomics | Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic Analysis Pubmed: 22064513 |
Clinical Cancer Research | Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancer Pubmed: 24046070 |
Onco Targets Ther | The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells PubMed: 25914546 |
Oncogene | Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanism Pubmed:29921847 |
Molecular Oncology | IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancer Pubmed:29465809 |
Cancers | Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver … Pubmed: 32443439 |
International Journal of Cancer | Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancer Pubmed:35689436 |