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Recombinant Hedgehog Homolog, Sonic (SHH)

HHG1; HLP3; HPE3; SMMCI; SHHN; SHH-N

  • Product No.RPB831Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Secreted
  • Predicted Molecular Mass22.0kDa
  • Accurate Molecular Mass20kDa(Analysis of differences refer to the manual)
  • Residues & TagsSer237~His428 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point6.35
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 184  US$ 460  US$ 920  US$ 2760  US$ 6900 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
United States Patent Application Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html
Annals of the Rheumatic Diseases Elevated serum levels of sonic hedgehog are associated with fibrotic and vascular manifestations in systemic sclerosis pubmed:28495672
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB831Mu01 Recombinant Hedgehog Homolog, Sonic (SHH) Positive Control; Immunogen; SDS-PAGE; WB.
APB831Mu01 Active Hedgehog Homolog, Sonic (SHH) Cell culture; Activity Assays.
PAB831Mu01 Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC; IP.
LAB831Mu71 Biotin-Linked Polyclonal Antibody to Hedgehog Homolog, Sonic (SHH) WB; IHC; ICC.