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Recombinant Neprilysin (CD10)

NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase

  • Product No.RPB785Ra02
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Chromosome
  • Predicted Molecular Mass46.6kDa
  • Accurate Molecular Mass47kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla286~Val428 with N-terminal GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point7.2
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 206  US$ 516  US$ 1032  US$ 3096  US$ 7740 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
13 Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors Ahajournals:Source
Biol Pharm Bull Effect of High Glucose Levels on Amyloid β Production in Retinas of Spontaneous Diabetes Mellitus Otsuka Long-Evans Tokushima Fatty Rats PubMed: 25832640
Eur J Heart Fail.  Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction Pubmed:26725876
8 A Test in Context: Neprilysin: Function, Inhibition, and Biomarker. pubmed:27491909
European Journal of Heart Failure Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study Pubmed: 30520545
European Journal of Heart Failure Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure Pubmed: 30828920
Clinical Research in Cardiology Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD) Pubmed: 32002632
Neurotoxicology Involvement of hippocampal agmatine in β1-42 amyloid induced memory impairment, neuroinflammation and BDNF signaling disruption in mice Pubmed: 32522471
European Heart Journal P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction
Clin Biochem Finding a reliable assay for soluble neprilysin Pubmed:35331754
Scientific Reports The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in …
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB785Ra02 Recombinant Neprilysin (CD10) Positive Control; Immunogen; SDS-PAGE; WB.
RPB785Ra01 Recombinant Neprilysin (CD10) Positive Control; Immunogen; SDS-PAGE; WB.
PAB785Ra01 Polyclonal Antibody to Neprilysin (CD10) WB; IHC; ICC; IP.
PAB785Ra02 Polyclonal Antibody to Neprilysin (CD10) WB; IHC; ICC; IP.
LAB785Ra71 Biotin-Linked Polyclonal Antibody to Neprilysin (CD10) WB; IHC; ICC.
SEB785Ra ELISA Kit for Neprilysin (CD10) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB785Ra Multiplex Assay Kit for Neprilysin (CD10) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.