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Recombinant Perforin 1 (PRF1)

FLH2; HPLH2; P1; PFP; Pore Forming Protein; Cytolysin; Lymphocyte pore-forming protein

  • Product No.RPB317Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Secreted, Cytoplasm, Endosome
  • Predicted Molecular Mass63.4kDa
  • Accurate Molecular Mass62kDa(Analysis of differences refer to the manual)
  • Residues & TagsLys32~Phe316 with N-terminal His and GST Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point7.0
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 240  US$ 600  US$ 1200  US$ 3600  US$ 9000 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Diagnostic Microbiology and Infectious Disease Granzyme B as a diagnostic marker of tuberculosis in patients with and without HIV coinfection Pubmed:26915636
FASEB JOURNAL The immunoregulatory effects of CD8 T-cell–derived perforin on diet-induced nonalcoholic steatohepatitis Pubmed: 30951375
Theranostics A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors Pubmed: 31695790
Mol Immunol CD317 mediates immunocytolysis resistance by RICH2/cytoskeleton-dependent membrane protection Pubmed: 33223223
INVESTIGATIONAL NEW DRUGS Dual-function chimeric antigen receptor T cells targeting c-Met and PD-1 exhibit potent anti-tumor efficacy in solid tumors Pubmed: 32772342
Am J Transl Res Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma 34377237
Nat Biomed Eng Cancer-cell stiffening via cholesterol depletion enhances adoptive T-cell immunotherapy 34873307
J Exp Med Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Pubmed:35061012
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB317Hu02 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Hu01 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
RPB317Hu01 Recombinant Perforin 1 (PRF1) Positive Control; Immunogen; SDS-PAGE; WB.
APB317Hu02 Active Perforin 1 (PRF1) Cell culture; Activity Assays.
PAB317Hu01 Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
LAB317Hu81 FITC-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IF.
LAB317Hu71 Biotin-Linked Polyclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC.
MAB317Hu22 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
MAB317Hu21 Monoclonal Antibody to Perforin 1 (PRF1) WB; IHC; ICC; IP.
SEB317Hu ELISA Kit for Perforin 1 (PRF1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB317Hu Multiplex Assay Kit for Perforin 1 (PRF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.