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Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL)

BALP; BSAP; HOPS; AP-TNAP; TNSALP; Bone Alkaline Phosphatase; Alkaline Phosphatase,Tissue-Nonspecific Isozyme; Alkaline Phosphatase, Liver/Bone/Kidney

  • Product No.RPB091Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass36.9kDa
  • Accurate Molecular Mass37kDa(Analysis of differences refer to the manual)
  • Residues & TagsPhe18~Arg335 with N-terminal His Tag
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point8.1
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Journal of Materials Science: Materials in Medicine Osteopenic bone cell response to strontium-substituted hydroxyapatite SpringerLink: n8233337w171v451
Osteoporosis International Dextromethorphan inhibits osteoclast differentiation by suppressing RANKL-induced nuclear factor-κB activation PubMed: 23400250
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice Wiley: Source
Acta Pharmacologica Sinica The antidepressant bupropion exerts alleviating properties in an ovariectomized osteoporotic rat model Pubmed:25544359
Biomedical Materials Enhanced differentiation of osteoblastic cells on novel chitosan/β-1, 3-glucan/bioceramic scaffolds for bone tissue regeneration Pubmed:25586067
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy PubMed: 25871861
J Biomed Mater Res A. New method for the fabrication of highly osteoconductive β-1,3-glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological characterization. Pubmed:27239050
J Bone Miner Metab MiR-142-5p promotes bone repair by maintaining osteoblast activity Pubmed:27085967
Journal of Biomedical Materials Research New method for the fabrication of highly osteoconductive β‐1, 3‐glucan/HA scaffold for bone tissue engineering: Structural, mechanical, and biological … doi:10.1002
Journal of Bone and Mineral Metabolism MiR‑142‑5p promotes bone repair by maintaining osteoblast activity pubmed:27085967
Scientific Reports Hydrolysis of Extracellular Pyrophosphate increases in post-hemodialysis plasma Pubmed:30038263
International Journal of Molecular Sciences  Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo Pubmed:29562730
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats Pubmed:29898457
Experimental and Therapeutic Medicine Combination therapy with BMP‑2 and psoralen enhances fracture healing in ovariectomized mice 10.3892:etm.2018.6353
American Journal of Translational Research Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9
The effect of low temperature atmospheric nitrogen plasma on MC3T3-E1 preosteoblast proliferation and differentiation in vitro
International Journal of Biomaterials Bovine Hydroxyapatite-Based Bone Scaffold with Gentamicin Accelerates Vascularization and Remodeling of Bone Defect 34104195
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPB091Ra01 Recombinant Alkaline Phosphatase, Tissue-nonspecific (ALPL) Positive Control; Immunogen; SDS-PAGE; WB.
PAB091Ra01 Polyclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) WB; IHC; ICC; IP.
MAB091Ra21 Monoclonal Antibody to Alkaline Phosphatase, Tissue-nonspecific (ALPL) WB; IHC; ICC; IP.
SEB091Ra ELISA Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB091Ra CLIA Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) Chemiluminescent immunoassay for Antigen Detection.
LMB091Ra Multiplex Assay Kit for Alkaline Phosphatase, Tissue-nonspecific (ALPL) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.