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Recombinant Midkine (MK)

MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2

  • Product No.RPA631Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass18.9kDa
  • Accurate Molecular Mass18kDa(Analysis of differences refer to the manual)
  • Residues & TagsAla22~Asp140 with N-terminal His Tag
  • Buffer Formulation100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 95%
  • Isoelectric Point9.3
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 168  US$ 420  US$ 840  US$ 2520  US$ 6300 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Midkine: From Embryogenesis to Pathogenesis and Therapy Immunochemical Methods for the Detection of Midkine in Samples of Human Origin Springer: Source
33 Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … 10.1016:j.ebiom.2017.11.020
Circulation Journal Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice pubmed:28781288
EBioMedicine Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … Pubmed:29233575
Journal of Clinical Laboratory Analysis Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall … Pubmed:29797475
Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese :
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells Pubmed: 33206337
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA631Mu01 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
RPA631Mu02 Recombinant Midkine (MK) Positive Control; Immunogen; SDS-PAGE; WB.
PAA631Mu01 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
PAA631Mu02 Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IP.
LAA631Mu71 Biotin-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC.
LAA631Mu81 FITC-Linked Polyclonal Antibody to Midkine (MK) WB; IHC; ICC; IF.
SEA631Mu ELISA Kit for Midkine (MK) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA631Mu Multiplex Assay Kit for Midkine (MK) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA631Mu01 ELISA Kit DIY Materials for Midkine (MK) Main materials for "Do It (ELISA Kit) Yourself".