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Recombinant Enolase, Neuron Specific (NSE)

ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase

  • Product No.RPA537Hu01
  • Organism SpeciesHomo sapiens (Human) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationMembrane, Cytoplasm
  • Predicted Molecular Mass49.0kDa
  • Accurate Molecular Mass50kDa(Analysis of differences refer to the manual)
  • Residues & TagsMet1~Leu434 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point5.1
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 140  US$ 350  US$ 700  US$ 2100  US$ 5250 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Neurotrauma The Anti-Inflammatory and Neuroprotective Effects of Ghrelin in Subarachnoid Hemorrhage-Induced Oxidative Brain Damage in Rats Liebert: 20091210
20 Melatonin treatment protects against spinal cord injury induced functional and biochemical changes in rat urinary bladder Wiley: source
Journal of Pharmacology and Toxicology Ginkgo biloba Enhances the Anticonvulsant and Neuroprotective Effects of Sodium Valproate Against Kainic Acid-induced Seizures in Mice Medwell: 2011679690
Addiction Biology Comparative gene expression profiling analysis of lymphoblastoid cells reveals neuron‐specific enolase gene (ENO2) as a susceptibility gene of heroin dependence PubMed: 21995595
Placenta Advanced glycation end products as an upstream molecule triggers ROS-induced sFlt-1 production in extravillous trophoblasts: A novel bridge between oxidative stress and preeclampsia Pubmed: 24144948
J Ethnopharmacol. Therapeutic time window and underlying therapeutic mechanism of breviscapine injection against cerebral ischemia/reperfusion injury in rats. Pubmed: 24291152
J Neurosurg. Detection of β-amyloid oligomers as a predictor of neurological outcome after brain injury. Pubmed: 23540266
Analytical Methods A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer read out Rsc: Source
Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits Pubmed:25111247
Experimental &amp; Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns Pubmed:25358349
Neuroscience Letters Preconditioning effect of (S)-3,5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague-Dawley rats. Pubmed:25562631
中华临床医师杂志 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 Journal: Fulltext
Am J Trop Med Hyg Blood–Brain Barrier Function and Biomarkers of Central Nervous System Injury in Rickettsial versus Other Neurological Infections in Laos PubMed: 26055741
Neurosci Lett Preconditioning effect of (S)-3, 5-dihydroxyphenylglycine on ischemic injury in middle cerebral artery occluded Sprague–Dawley rats PubMed: 25562631
Physiol Rep S100B and NSE serum concentrations after simulated diving in rats PubMed: 26462746
Int J Mol Sci The effect of electroporation of a lyotroic liquid crystal Genistein-based formulation in the recovery of murine melanoma lesions PubMed: 26184156
PLoS One Isoflurane Post-Treatment Improves Outcome after an Embolic Stroke in Rabbits PubMed: 26645542
Фармакология, клиническая фармакология Волгоградский государственный медицинский университет uploads:dsovet
Analytical Methods Synthesis of sunflower-like gold nanostructures and their application in electrochemical immunoassays using the nanogold-triggered hydrogen evolution reaction articlelanding:c5ay03174e
中国临床药理学与治疗学 右美托咪定对脑膜瘤切除术患者血清S100β 蛋白及神经元特异性烯醇化酶的影响 article:9794
Asian Pacific Journal of Tropical Medicine Perfusion of gastrodin in abdominal aorta for alleviating spinal cord ischemia reperfusion injury science:S1995764516300955
Izvorni znanstveni članak SERUMSKA RAZINA ZA NEURON SPECIFIČNE ENOLAZE KAO BILJEG ISHEMIJSKO-REPERFUZIJSKOG OŠTEĆENJA U BOLESNIKA PODVRGNUTIH KAROTIDNOJ ENDARTEREKTOMIJI 262238
Neurourol Urodyn Functional and structural changes of the urinary bladder following spinal cord injury; treatment with alpha lipoic acid pubmed:27490041
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830
cellular physiology and biochemistry Hydrogen-Rich Saline Attenuates Brain Injury Induced by Cardiopulmonary Bypass and Inhibits Microvascular Endothelial Cell Apoptosis Via the PI3K/Akt/GSK3β … pubmed:29040978
Brain Research Intra-arterial human urinary kallidinogenase alleviates brain injury in rats with permanent middle cerebral artery occlusion through PI3K/AKT/FoxO1 signaling pathway Pubmed:29510144
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336
Journal of the Neurological Sciences S100B, NSE and MMP-9 fail to predict neurologic outcome while elevated S100B associates with milder initial clinical presentation after aneurysmal subarachnoid … Pubmed:29801873
Iranian Journal of Veterinary Research Nitrosative stress indices in dogs with neurological form of canine distemper Doi: 10.22099/IJVR.2018.4943
Evidence-Based Complementary and Alternative Medicine Naomaitai Ameliorated Brain Damage in Rats with Vascular Dementia by PI3K/PDK1/AKT Signaling Pathway
The Eurasian Journal of Medicine Effects of Conivaptan versus Mannitol on Post-Ischemic Brain Injury and Edema
Medicina The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the …
ACTA NEUROBIOLOGIAE EXPERIMENTALIS Neuroprotective effect of poly (lactic-co-glycolic acid) nanoparticle-bound brain-derived neurotrophic factor in a permanent middle cerebral artery occlusion … Pubmed: 32214270
Stem Cells International Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2 … Pubmed: 32300366
European Review for Medical and Pharmacological Sciences Neuroprotective effect of CTRP3 overexpression against sevoflurane anesthesia-induced cognitive dysfunction in aged rats through activating AMPK/SIRT1 and … Pubmed: 32432773
Neurobiology of Learning and Memory Effects of Circadian Rhythm Disorder on the Hippocampus of SHR and WKY Rats
The role of light desynchronosis in the development of stress-induced aging
ИЗУЧЕНИЕ ЦЕРЕБРОПРОТЕКТОРНОГО ДЕЙСТВИЯ ПРЕПАРАТОВ РАЗЛИЧНЫХ ФАРМАКОЛОГИЧЕСКИХ ГРУПП В УСЛОВИЯХ …
Chinese Journal of Analytical Chemistry Investigation of Library Input on Aptamers Selection Efficiency Using Capillary Electrophoresis
Ther Adv Chronic Dis The effect of doxycycline on neuron-specific enolase in patients with traumatic brain injury: a randomized controlled trial 34262678
J Surg Res Perillaldehyde Alleviates Spinal Cord Ischemia-Reperfusion Injury Via Activating the Nrf2 Pathway 34399353
Cephalalgia Evaluation of serum neuron specific enolase levels among patients with primary and secondary burning mouth syndrome 34644195
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SEA537Hu ELISA Kit for Enolase, Neuron Specific (NSE) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA537Hu01 ELISA Kit DIY Materials for Enolase, Neuron Specific (NSE) Main materials for "Do It (ELISA Kit) Yourself".
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