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Recombinant Tissue Plasminogen Activator (tPA)

PLAT; T-PA; t-plasminogen activator; Alteplase; Reteplase

  • Product No.RPA525Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted, Extracellular matrix
  • Predicted Molecular Mass23.6kDa
  • Accurate Molecular Mass33kDa(Analysis of differences refer to the manual)
  • Residues & TagsGly311~Asn487B with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 90%
  • Isoelectric Point6.2
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 184  US$ 460  US$ 920  US$ 2760  US$ 6900 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Annals of Clinical & Laboratory Science Alteration of Hemostatic Parameters in Patients with Different Levels of Subclinical Hypothyroidism and the Effect of L-thyroxine Treatment pubmed:28249913
Cellular Physiology and Biochemistry Defective Platelet Activation and Bleeding Complications upon Cholestasis in Mice pubmed:28441661
Journal of allergy and clinical immunology Cytokine and estrogen stimulation of endothelial cells augments activation of the prekallikrein-high molecular weight kininogen complex: Implications for hereditary angioedema. pubmed:27826093
European Journal of Neuroscience Urokinase receptor and tissue plasminogen activator as immediate‐early genes in pentylenetetrazole‐induced seizures in the mouse brain Pubmed: 31587391
Phytomedicine Sodium aescinate significantly suppress postoperative peritoneal adhesion by inhibiting the RhoA/ROCK signaling pathway Pubmed: 32120245
Sci Rep Fibroblast growth factor-21 as a novel metabolic factor for regulating thrombotic homeostasis Pubmed:35013379
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA525Ra01 Recombinant Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
EPA525Ra61 Eukaryotic Tissue Plasminogen Activator (tPA) Positive Control; Immunogen; SDS-PAGE; WB.
PAA525Ra01 Polyclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
MAA525Ra21 Monoclonal Antibody to Tissue Plasminogen Activator (tPA) WB; IHC; ICC; IP.
SEA525Ra ELISA Kit for Tissue Plasminogen Activator (tPA) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA525Ra Multiplex Assay Kit for Tissue Plasminogen Activator (tPA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.