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Recombinant Collagen Type I Alpha 2 (COL1a2)

COL1-A2; OI4; Collagen Alpha-2(I)chain; osteogenesis imperfecta type IV; Collagen Of Skin,Tendon And bone,Alpha-2 Chain

  • Product No.RPA215Ra01
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceProkaryotic expression
  • HostE.coli
  • Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
  • Subcellular LocationSecreted
  • Predicted Molecular Mass33.2kDa
  • Accurate Molecular Mass33kDa(Analysis of differences refer to the manual)
  • Residues & TagsVal1109~Lys1372 with N-terminal His Tag
  • Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Traits Freeze-dried powder
  • Purity> 97%
  • Isoelectric Point5.8
  • Applications Positive Control; Immunogen; SDS-PAGE; WB.
    If bio-activity of the protein is needed, please check active protein.
  • DownloadInstruction Manual
  • UOM 10µg50µg 200µg 1mg 5mg
  • FOB US$ 180  US$ 450  US$ 900  US$ 2700  US$ 6750 
    For more details, please contact local distributors!

SEQUENCE

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Plos one Inhibition of TGF-β Signaling Enables Human Corneal Endothelial Cell Expansion In Vitro for Use in Regenerative Medicine PubMed: PMC3581499
J Proteomics Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC) PubMed: 26718731
PLoS One Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis pubmed:28665955
American Journal of Translational Research A novel hybrid 3D-printed titanium scaffold for osteogenesis in a rabbit calvarial defect model Pubmed:29511441
Sci Rep The effect of hypoxia on the proteomic signature of pig adipose-derived stromal/stem cells (pASCs) Pubmed: 33208768
Experimental and Therapeutic Medicine Protective effects of halophyte complex extract against UVB?induced damage in human keratinocytes and the skin of hairless mice 33986847
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA215Ra01 Recombinant Collagen Type I Alpha 2 (COL1a2) Positive Control; Immunogen; SDS-PAGE; WB.
APA215Ra01 Active Collagen Type I Alpha 2 (COL1a2) Cell culture; Activity Assays.
LAA215Ra71 Biotin-Linked Polyclonal Antibody to Collagen Type I Alpha 2 (COL1a2) WB; IHC; ICC.
SEA215Ra ELISA Kit for Collagen Type I Alpha 2 (COL1a2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA215Ra Multiplex Assay Kit for Collagen Type I Alpha 2 (COL1a2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.