Packages (Simulation)

Polyclonal Antibody to Spondin 2 (SPON2)

Image (I)

DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 20μg/ml Rabbit Anti-Rat SPON2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
DAB staining on IHC-P;
Samples: Rat Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat SPON2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

WB Image

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Instruction Manual

Polyclonal-Antibody-to-Spondin-2--SPON2--PAF396Ra01.pdf

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Polyclonal Antibody to Spondin 2 (SPON2)

DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1

Product No.PAF396Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen n/a
Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
TraitsLiquid
Concentration500µg/mL
Organism Species Moren/a
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 114 266 US$ 266 US$ 380 US$ 950 US$ 3800
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against SPON2. It has been selected for its ability to recognize SPON2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine	Citations
The Journal of Urology	Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancer Pubmed: 23665271
Oxford Journals	SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASES Content: 30
Nephrology (Carlton)	Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic Nephropathy PubMed: 25973958
Oncogene	The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation pubmed:28991232
Endocrinology	Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development Pubmed: 30715257
Journal of Cellular and Molecular Medicine	Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice Pubmed: 32614521

Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPF396Ra01	Recombinant Spondin 2 (SPON2)	Positive Control; Immunogen; SDS-PAGE; WB.
PAF396Ra01	Polyclonal Antibody to Spondin 2 (SPON2)	WB; IHC; ICC; IP.
MAF396Ra21	Monoclonal Antibody to Spondin 2 (SPON2)	WB; IHC; ICC; IP.
SEF396Ra	ELISA Kit for Spondin 2 (SPON2)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMF396Ra	Multiplex Assay Kit for Spondin 2 (SPON2) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.