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Monoclonal Antibody to Spondin 2 (SPON2)

DIL1; Mindin; M-spondin; Extracellular Matrix Protein; Differentially expressed in cancerous and non-cancerous lung cells 1

  • Product No.MAF396Ra21
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • SourceMonoclonal antibody preparation
  • HostMouse
  • Potency (Clone Number)n/a
  • Ig Isotype IgG
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration1mg/mL
  • Organism Species Moren/a
  • ApplicationsWB; IHC; ICC; IP.
    If the antibody is used in flow cytometry, please check FCM antibodies.
  • Downloadn/a
  • UOM 20µl100µl 200µl 1ml 10ml
  • FOB US$ 120  US$ 280  US$ 400  US$ 1000  US$ 4000 
    For more details, please contact local distributors!

SPECIFITY

The antibody is a mouse monoclonal antibody raised against SPON2. It has been selected for its ability to recognize SPON2 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:500-5000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The Journal of Urology Spondin-2, a secreted extracellular matrix protein, is a novel diagnostic biomarker for prostate cancer Pubmed: 23665271
Oxford Journals SP136EVALUATION OF SERUM SPONDIN 2 LEVELS IN THE DIFFERENT ETIOLOGIES OF GLOMERULAR DISEASES Content: 30
Nephrology (Carlton) Evaluation of Serum Spondin 2 Levels in the Different Stages of Type 2 Diabetic Nephropathy PubMed: 25973958
Oncogene The extracellular matrix protein mindin attenuates colon cancer progression by blocking angiogenesis via Egr-1-mediated regulation pubmed:28991232
Endocrinology Study of human T21 placenta suggests a potential role of mesenchymal spondin-2 in placental vascular development Pubmed: 30715257
Journal of Cellular and Molecular Medicine Mindin serves as a tumour suppressor gene during colon cancer progression through MAPK/ERK signalling pathway in mice Pubmed: 32614521
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPF396Ra01 Recombinant Spondin 2 (SPON2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF396Ra01 Polyclonal Antibody to Spondin 2 (SPON2) WB; IHC; ICC; IP.
MAF396Ra21 Monoclonal Antibody to Spondin 2 (SPON2) WB; IHC; ICC; IP.
SEF396Ra ELISA Kit for Spondin 2 (SPON2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF396Ra Multiplex Assay Kit for Spondin 2 (SPON2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.