Polyclonal Antibody to Acid Sphingomyelinase (ASM)

SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase

Product No.PAB360Hu01
Organism SpeciesHomo sapiens (Human) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPB360Hu01-Recombinant Acid Sphingomyelinase (ASM)
Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
TraitsLiquid
Concentration0.5mg/mL
Organism Species MoreMus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 100 232 US$ 232 US$ 332 US$ 830 US$ 3320
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against ASM. It has been selected for its ability to recognize ASM in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine	Citations
Blood.?	Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126
Journal of Clinical Lipidology	Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007
Diabetes & metabolism	Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696
INFLAMMATION	Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756
Indian J Ophthalmol	Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809

Catalog No.	Related products for research use of Homo sapiens (Human) Organism species	Applications (RESEARCH USE ONLY!)
EPB360Hu61	Eukaryotic Acid Sphingomyelinase (ASM)	Positive Control; Immunogen; SDS-PAGE; WB.
RPB360Hu01	Recombinant Acid Sphingomyelinase (ASM)	Positive Control; Immunogen; SDS-PAGE; WB.
APB360Hu61	Active Acid Sphingomyelinase (ASM)	Cell culture; Activity Assays.
PAB360Hu01	Polyclonal Antibody to Acid Sphingomyelinase (ASM)	WB; IHC; ICC; IP.
MAB360Hu22	Monoclonal Antibody to Acid Sphingomyelinase (ASM)	WB; IHC; ICC; IP.
SEB360Hu	ELISA Kit for Acid Sphingomyelinase (ASM)	Enzyme-linked immunosorbent assay for Antigen Detection.
MEB360Hu	Mini Samples ELISA Kit for Acid Sphingomyelinase (ASM)	Enzyme-linked immunosorbent assay for Antigen Detection.
LMB360Hu	Multiplex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay)	FLIA Kit for Antigen Detection.