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Multiplex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay)
SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase
(Note: Up to 8-plex in one testing reaction)
- Product No.LMB360Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3.5h
- Detection Range0.01-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.003 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 415
US$ 431
US$ 455
US$ 487
US$ 519
US$ 567
US$ 638
Result
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Specificity
This assay has high sensitivity and excellent specificity for detection of Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 78-101 | 84 |
EDTA plasma(n=5) | 95-103 | 98 |
heparin plasma(n=5) | 98-105 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 99-105% | 91-98% | 83-92% | 81-91% |
EDTA plasma(n=5) | 88-95% | 82-97% | 79-89% | 98-105% |
heparin plasma(n=5) | 90-104% | 90-103% | 98-105% | 83-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
96-well plate | 1 | Plate sealer for 96 wells | 4 |
Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
Pre-Mixed Magnetic beads (22#:ASM) | 1 | Analysis buffer | 1×20mL |
Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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Magazine | Citations |
Blood.? | Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126 |
Journal of Clinical Lipidology | Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007 |
Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696 |
INFLAMMATION | Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756 |
Indian J Ophthalmol | Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
EPB360Hu61 | Eukaryotic Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB360Hu01 | Recombinant Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB360Hu61 | Active Acid Sphingomyelinase (ASM) | Cell culture; Activity Assays. |
PAB360Hu01 | Polyclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
MAB360Hu22 | Monoclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
SEB360Hu | ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEB360Hu | Mini Samples ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB360Hu | Multiplex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |