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ELISA Kit for Acid Sphingomyelinase (ASM)
SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1,Acid Lysosomal; Simply Sphingomyelinase
- Product No.SEB360Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range0.156-10ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.057ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
- FOB
US$ 466
For more details, please contact local distributors! US$ 665 US$ 2993 US$ 5653 US$ 46550
Specificity
This assay has high sensitivity and excellent specificity for detection of Acid Sphingomyelinase (ASM).
No significant cross-reactivity or interference between Acid Sphingomyelinase (ASM) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Acid Sphingomyelinase (ASM) and the recovery rates were calculated by comparing the measured value to the expected amount of Acid Sphingomyelinase (ASM) in samples.
Matrix | Recovery range (%) | Average(%) |
serum(n=5) | 96-104 | 101 |
EDTA plasma(n=5) | 97-104 | 101 |
heparin plasma(n=5) | 78-104 | 101 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acid Sphingomyelinase (ASM) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acid Sphingomyelinase (ASM) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Acid Sphingomyelinase (ASM) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-105% | 82-99% | 80-96% | 97-104% |
EDTA plasma(n=5) | 91-101% | 86-101% | 87-102% | 88-102% |
heparin plasma(n=5) | 95-103% | 89-102% | 83-101% | 84-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
Reagents | Quantity | Reagents | Quantity |
Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
Standard | 2 | Standard Diluent | 1×20mL |
Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
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Magazine | Citations |
Blood.? | Acid sphingomyelinase is activated in sickle cell erythrocytes and contributes to inflammatory microparticle generation in SCD Pubmed:25075126 |
Journal of Clinical Lipidology | Increase in acid sphingomyelinase level in human retinal endothelial cells and CD34+ circulating angiogenic cells isolated from diabetic individuals is associated with dysfunctional retinal vasculature and vascular repair process in diabetes 10.1016/j.jacl.2017.03.007 |
Diabetes & metabolism | Fenofibrate decreases plasma ceramide in type 2 diabetes patients: A novel marker of CVD? pubmed:28499696 |
INFLAMMATION | Acid Sphingomyelinase and Acid ¦Â-Glucosidase 1 Exert Opposite Effects on Interleukin-1¦Â-Induced Interleukin 6 Production in Rheumatoid Arthritis Fibroblast-Like Synoviocytes 33665756 |
Indian J Ophthalmol | Evaluation of sphingolipid metabolism on diabetic retinopathy 34708809 |
Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
EPB360Hu61 | Eukaryotic Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB360Hu01 | Recombinant Acid Sphingomyelinase (ASM) | Positive Control; Immunogen; SDS-PAGE; WB. |
APB360Hu61 | Active Acid Sphingomyelinase (ASM) | Cell culture; Activity Assays. |
PAB360Hu01 | Polyclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
MAB360Hu22 | Monoclonal Antibody to Acid Sphingomyelinase (ASM) | WB; IHC; ICC; IP. |
SEB360Hu | ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEB360Hu | Mini Samples ELISA Kit for Acid Sphingomyelinase (ASM) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB360Hu | Multiplex Assay Kit for Acid Sphingomyelinase (ASM) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |