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Western Blot; Sample: Human Leukocyte lysate Primary Ab: 2μg/ml Mouse Anti-Human S100A9 Antibody Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9)
MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain
- Product No.MAB793Hu29
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)H9
- Ig Isotype IgG1 Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPB793Hu01-Recombinant S100 Calcium Binding Protein A9 (S100A9)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 109
US$ 253
US$ 362
US$ 905
US$ 3620
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against S100A9. It has been selected for its ability to recognize S100A9 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| J. Proteome Res. | Differential Proteomic Analysis Distinguishes Tissue Repair Biomarker Signatures in Wound Exudates Obtained from Normal Healing and Chronic Wounds Pubmed: source |
| Biochemical and Biophysical Research Communications | High circulating levels of S100A8/A9 complex (calprotectin) in male Japanese with abdominal adiposity and dysregulated expression of S100A8 and S100A9 in adipose tissues of obese mice ScienceDirect: S0006291X1200352X |
| PLoS One. | Proteins with Altered Levels in Plasma from Glioblastoma Patients as Revealed by iTRAQ-Based Quantitative Proteomic Analysis PubMed: 23029420 |
| Mainz | Identifizierung neuer potentieller Biomarker für das Kolonkarzinom sowie dessen Vorstufen Info:Source |
| PLOS ONE | Susceptibility to COPD: Differential Proteomic Profiling after Acute Smoking Pubmed:25036363 |
| the faseb journal | Soluble receptor for advanced glycation end products inhibits disease progression in autosomal dominant polycystic kidney disease by down-regulating cell proliferation PubMed: 25934702 |
| Sci Rep. | S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine pmc:PMC4810364 |
| Journal of Alzheimer's Disease | Proteomics Analysis of Blood Serums from Alzheimer's Disease Patients Using iTRAQ LabelingTechnology. pubmed:27911324 |
| International journal of molecular sciences | Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds. pubmed:28672877 |
| Gastroenterology | Tu1938-Micronutrients Assessment in Patients with Cirrhosis 10.1016:S0016-5085(18)33544-3 |
| Gastroenterology | Tu1942-PI3K/AKT-Mediated Upregulation of C7 Inhibits Colorectal Cancer Proliferation and Metastasis 10.1016:S0016-5085(18)33544-5 |
| Journal of Biological Chemistry | Signaling between pancreatic β-cells and macrophages via S100 calcium-binding protein A8 exacerbates β-cell apoptosis and islet inflammation Pubmed:29496993 |
| Cancer Management and Research | The role of calgranulin B gene on the biological behavior of squamous cervical cancer in vitro and in vivo Pubmed:29497331 |
| European review for medical and pharmacological sciences | S100A8/A9 promotes MMP-9 expression in the fibroblasts from cardiac rupture after myocardial infarction by inducing macrophages secreting TNFα. Pubmed:29949169 |
| Estudio de células inmunosupresoras y biomarcadores circulantes en carcinoma renal | |
| Lung | Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis Pubmed: 31115649 |
| 中国医药导报 | 降钙素原, 超敏C 反应蛋白, 红细胞沉降率及S100A9 对肺结核合并肺部细菌感染的影响 |
| scandinavian journal of clinical & laboratory investigation | Serum S100A6, S100A8, S100A9 and S100A11 proteins in colorectal neoplasia: results of a single centre prospective study Pubmed: 31856598 |
| PLoS One | Adverse reproductive effects of S100A9 on bovine sperm and early embryonic development in vitro Pubmed: 31945120 |
| Sci Rep | Overexpression of S100A9 in tumor stroma contribute to immune evasion of NK/T cell lymphoma and predict poor response rate 34045609 |
| university of copenhagen | Adjunctive S100A8/A9 Immunomodulation Hinders Cipro fl oxacin Resistance in |