≡

S100 Calcium Binding Protein A9 (S100A9)

[Edit]

MRP14; MIF; 60B8AG; CFAG; NIF; CAGB; CGLB; L1AG; P14; Calgranulin B; Migration inhibitory factor-related protein 14; Calprotectin L1H subunit; Leukocyte L1 complex heavy chain

S100 Calcium Binding Protein A9 (S100A9)
CCC B793
HGNC 10499
MGI 1338947
PubMed 22470535
RGD 620267
UniProt P06702
Wiki S100A9

S100 calcium binding protein A9 has been implicated in the abnormal differentiation of myeloid cells in the stroma of cancer. This contributes to creating an overall immunosuppressive microenvironemnt that may contribute to the inability of a protective or therapeutic cellular immune response to be generated by the tumor-bearing host.

Outside of malignancy, S100A9 in association with its dimerization partner, S100A8 (MRP8 or calgranulin A) signals for lymphocyte recruitment in sites of inflammation. S100A9/A8 (synonyma: Calgranulin A/B; Calprotectin) are also regarded as marker proteins for a number of inflammatory diseases in humans, especially in rheumatoid arthritis.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB793Hu01 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
APB793Hu01 Active S100 Calcium Binding Protein A9 (S100A9) Cell culture; Activity Assays.
Antibodies PAB793Hu01 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
MAB793Hu22 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC
LAB793Hu82 FITC-Linked Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IF.
LAB793Hu72 Biotin-Linked Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC.
LAB793Hu71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC.
LAB793Hu81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IF.
MAB793Hu21 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC
MAB793Hu23 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB
MAB793Hu27 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
MAB793Hu24 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC
MAB793Hu25 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
MAB793Hu29 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
Assay Kits SEB793Hu ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB793Mu01 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
RPB793Mu02 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
APB793Mu01 Active S100 Calcium Binding Protein A9 (S100A9) Cell culture; Activity Assays.
Antibodies PAB793Mu01 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
LAB793Mu71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC.
LAB793Mu81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IF.
PAB793Mu02 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
Assay Kits SEB793Mu ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB793Ra01 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB793Ra01 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
LAB793Ra71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC.
Assay Kits SEB793Ra ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Canis familiaris; Canine (Dog)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB793Ca01 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB793Ca01 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
Assay Kits n/a CLIA Kit for S100 Calcium Binding Protein A9 (S100A9) CLIA Kit Customized Service Offer
n/a ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) ELISA Kit Customized Service Offer

Organism species: Bos taurus; Bovine (Cattle)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPB793Bo01 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
RPB793Bo02 Recombinant S100 Calcium Binding Protein A9 (S100A9) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAB793Bo01 Polyclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
MAB793Bo21 Monoclonal Antibody to S100 Calcium Binding Protein A9 (S100A9) WB; IHC; ICC; IP.
Assay Kits SEB793Bo ELISA Kit for S100 Calcium Binding Protein A9 (S100A9) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis."Nature 330:80-82(1987) [PubMed] [Europe PMC] [Abstract]
  2. "Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid differentiation."Mol. Cell. Biol. 8:2402-2410(1988) [PubMed] [Europe PMC] [Abstract]
  3. "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases."J. Biol. Chem. 264:8356-8360(1989) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and biological annotation of human chromosome 1." Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Ionomycin-regulated phosphorylation of the myeloid calcium-binding protein p14."Nature 342:189-192(1989) [PubMed] [Europe PMC] [Abstract]
  7. "Amino acid sequences of 60B8 antigens induced in HL-60 cells by 1,25-dihydroxyvitamin D3. The antigens are identical with macrophage-related protein-14 and -8."Chem. Pharm. Bull. 37:1576-1580(1989) [PubMed] [Europe PMC] [Abstract]
  8. "Microsequences of 145 proteins recorded in the two-dimensional gel protein database of normal human epidermal keratinocytes."Electrophoresis 13:960-969(1992) [PubMed] [Europe PMC] [Abstract]
  9. "In vitro antimicrobial activity of the human neutrophil cytosolic S-100 protein complex, calprotectin, against Capnocytophaga sputigena."J. Dent. Res. 72:517-523(1993) [PubMed] [Europe PMC] [Abstract]
  10. "Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway."J. Biol. Chem. 272:9496-9502(1997) [PubMed] [Europe PMC] [Abstract]
  11. "The microbial receptor CEACAM3 is linked to the calprotectin complex in granulocytes."Biochem. Biophys. Res. Commun. 289:191-197(2001) [PubMed] [Europe PMC] [Abstract]
  12. "Proinflammatory activities of S100: proteins S100A8, S100A9, and S100A8/A9 induce neutrophil chemotaxis and adhesion."J. Immunol. 170:3233-3242(2003) [PubMed] [Europe PMC] [Abstract]
  13. "MRP8 and MRP14 control microtubule reorganization during transendothelial migration of phagocytes."Blood 104:4260-4268(2004) [PubMed] [Europe PMC] [Abstract]
  14. "Myeloid-related proteins 8 and 14 induce a specific inflammatory response in human microvascular endothelial cells."Blood 105:2955-2962(2005) [PubMed] [Europe PMC] [Abstract]
  15. "The arachidonic acid-binding protein S100A8/A9 promotes NADPH oxidase activation by interaction with p67phox and Rac-2."FASEB J. 19:467-469(2005) [PubMed] [Europe PMC] [Abstract]
  16. "Myeloid-related protein-14 is a p38 MAPK substrate in human neutrophils."J. Immunol. 174:7257-7267(2005) [PubMed] [Europe PMC] [Abstract]
  17. "Regulation of S100A8/A9 (calprotectin) binding to tumor cells by zinc ion and its implication for apoptosis-inducing activity."Mediators Inflamm. 2005:280-292(2005) [PubMed] [Europe PMC] [Abstract]
  18. "Interaction between S100A8/A9 and annexin A6 is involved in the calcium-induced cell surface exposition of S100A8/A9."J. Biol. Chem. 283:31776-31784(2008) [PubMed] [Europe PMC] [Abstract]
  19. "Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment."J. Proteome Res. 7:5167-5176(2008) [PubMed] [Europe PMC] [Abstract]
  20. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
  21. "Anti-infective protective properties of S100 calgranulins."Antiinflamm. Antiallergy Agents Med. Chem. 8:290-305(2009) [PubMed] [Europe PMC] [Abstract]
  22. "A novel p53 target gene, S100A9, induces p53-dependent cellular apoptosis and mediates the p53 apoptosis pathway."Biochem. J. 422:363-372(2009) [PubMed] [Europe PMC] [Abstract]
  23. "S100A8/A9: a Janus-faced molecule in cancer therapy and tumorgenesis."Eur. J. Pharmacol. 625:73-83(2009) [PubMed] [Europe PMC] [Abstract]
  24. "Substitution of methionine 63 or 83 in S100A9 and cysteine 42 in S100A8 abrogate the antifungal activities of S100A8/A9: potential role for oxidative regulation."FEMS Immunol. Med. Microbiol. 55:55-61(2009) [PubMed] [Europe PMC] [Abstract]
  25. "Calprotectin S100A9 calcium-binding loops I and II are essential for keratinocyte resistance to bacterial invasion."J. Biol. Chem. 284:7078-7090(2009) [PubMed] [Europe PMC] [Abstract]
  26. "The endogenous Toll-like receptor 4 agonist S100A8/S100A9 (calprotectin) as innate amplifier of infection, autoimmunity, and cancer."J. Leukoc. Biol. 86:557-566(2009) [PubMed] [Europe PMC] [Abstract]
  27. "Identification of human S100A9 as a novel target for treatment of autoimmune disease via binding to quinoline-3-carboxamides."PLoS Biol. 7:E97-E97(2009) [PubMed] [Europe PMC] [Abstract]
  28. "S100A8/A9 induces autophagy and apoptosis via ROS-mediated cross-talk between mitochondria and lysosomes that involves BNIP3."Cell Res. 20:314-331(2010) [PubMed] [Europe PMC] [Abstract]
  29. "S100 Calgranulins in inflammatory arthritis."Immunol. Cell Biol. 88:41-49(2010) [PubMed] [Europe PMC] [Abstract]
  30. "Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism."J. Leukoc. Biol. 87:905-914(2010) [PubMed] [Europe PMC] [Abstract]
  31. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
  32. "Inflammation-associated S100 proteins: new mechanisms that regulate function."Amino Acids 41:821-842(2011) [PubMed] [Europe PMC] [Abstract]
  33. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  34. "Damage-associated molecular pattern S100A9 increases bactericidal activity of human neutrophils by enhancing phagocytosis."J. Immunol. 186:3622-3631(2011) [PubMed] [Europe PMC] [Abstract]
  35. "S100A8 and S100A9 in cardiovascular biology and disease."Arterioscler. Thromb. Vasc. Biol. 32:223-229(2012) [PubMed] [Europe PMC] [Abstract]
  36. "Induction of nuclear factor-kappaB responses by the S100A9 protein is Toll-like receptor-4-dependent."Immunology 137:172-182(2012) [PubMed] [Europe PMC] [Abstract]
  37. "Dynamic mobility of immunological cells expressing S100A8 and S100A9 in vivo: a variety of functional roles of the two proteins as regulators in acute inflammatory reaction."Inflammation 35:409-419(2012) [PubMed] [Europe PMC] [Abstract]
  38. "Pro-inflammatory S100A8 and S100A9 proteins: self-assembly into multifunctional native and amyloid complexes."Int. J. Mol. Sci. 13:2893-2917(2012) [PubMed] [Europe PMC] [Abstract]
  39. "S100A8 and S100A9: new insights into their roles in malignancy."J. Innate Immun. 4:31-40(2012) [PubMed] [Europe PMC] [Abstract]
  40. "Molecular interface of S100A8 with cytochrome b and NADPH oxidase activation."PLoS ONE 7:E40277-E40277(2012) [PubMed] [Europe PMC] [Abstract]
  41. "MRP14 (S100A9) protein interacts with Alzheimer beta-amyloid peptide and induces its fibrillization."PLoS ONE 7:E32953-E32953(2012) [PubMed] [Europe PMC] [Abstract]
  42. "Constitutive neutrophil apoptosis: regulation by cell concentration via S100 A8/9 and the MEK-ERK pathway."PLoS ONE 7:E29333-E29333(2012) [PubMed] [Europe PMC] [Abstract]
  43. "Target-selective protein S-nitrosylation by sequence motif recognition."Cell 159:623-634(2014) [PubMed] [Europe PMC] [Abstract]
  44. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  45. "The crystal structure of human MRP14 (S100A9), a Ca(2+)-dependent regulator protein in inflammatory process."J. Mol. Biol. 316:265-276(2002) [PubMed] [Europe PMC] [Abstract]
  46. "The crystal structure of the human (S100A8/S100A9)2 heterotetramer, calprotectin, illustrates how conformational changes of interacting alpha-helices can determine specific association of two EF-hand proteins."J. Mol. Biol. 370:887-898(2007) [PubMed] [Europe PMC] [Abstract]