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Human HepG2 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human PRKAa2 Polyclonal Antibody (Catalog FAD357Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Anti-Protein Kinase, AMP Activated Alpha 2 (PRKAa2) Polyclonal Antibody
AMPK; AMPK2; PRKAA; 5'-AMP-Activated Protein Kinase Catalytic Subunit Alpha-2; Acetyl-CoA carboxylase kinase; Hydroxymethylglutaryl-CoA reductase kinase
- Product No.FAD357Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)n/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFCM
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
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For more details, please contact local distributors!
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPD357Hu01 | Recombinant Protein Kinase, AMP Activated Alpha 2 (PRKAa2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAD357Hu01 | Polyclonal Antibody to Protein Kinase, AMP Activated Alpha 2 (PRKAa2) | WB; IHC; ICC/IF |
| FAD357Hu01 | Anti-Protein Kinase, AMP Activated Alpha 2 (PRKAa2) Polyclonal Antibody | FCM |