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Human Hela cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human SMAD3 Polyclonal Antibody (Catalog FAC123Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Anti-SMAD family member 3 (SMAD3) Polyclonal Antibody
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- Product No.FAC123Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Potency (Clone Number)n/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
US$ 120
US$ 200
US$ 320
US$ 400
US$ 700
For more details, please contact local distributors!
USAGE
Flow cytometry:20µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| У¿¯ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC123Hu01 | Recombinant SMAD family member 3 (SMAD3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Polyclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| FAC123Hu01 | Anti-SMAD family member 3 (SMAD3) Polyclonal Antibody | Flow cytometry. |
| MAC123Hu21 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu22 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu23 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |