Packages (Simulation)
Image (I)
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DAB staining on IHC-P; Sample: Human Kidney Tissue; Primary Ab: 40µg/ml Mouse Anti-Human Smad3 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver Tissue;
Primary Ab: 40µg/ml Mouse Anti-Human Smad3 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
Image (II)
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Western Blot; Sample: Lane1: Porcine Pancreas lysate; Lane2: Jurkat cell lysate; Lane3: 293T cell lysate Primary Ab: 2μg/ml Mouse Anti-Human SMAD3 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
Quality Guarantee
Instruction Manual
Certificate
Monoclonal Antibody to SMAD family member 3 (SMAD3)
JV15-2; MADH3; Mothers Against Decapentaplegic Homolog 3
- Product No.MAC123Hu21
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourceMonoclonal antibody preparation
- HostMouse
- Potency (Clone Number)C8
- Ig Isotype IgG2b Kappa
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Immunogen RPC123Hu01-Recombinant SMAD family member 3 (SMAD3)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration1mg/mL
- Organism Species MoreSus scrofa; Porcine (Pig)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
- FOB
US$ 114
US$ 266
US$ 380
US$ 950
US$ 3800
For more details, please contact local distributors!
SPECIFITY
The antibody is a mouse monoclonal antibody raised against SMAD3. It has been selected for its ability to recognize SMAD3 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
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Antibody Labeling Customized Service
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Protein A/G Purification Column
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Staining Solution for Cells and Tissue
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Western Blot (WB) Experiment Service
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Immunohistochemistry (IHC) Experiment Service
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Immunocytochemistry (ICC) Experiment Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Long-arm Biotin Labeling Kit
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Real Time PCR Experimental Service
| Magazine | Citations |
| У¿¯ | Role of Angiotensin II and Renal Nerves on TGF-b/SmadPathway in Diabetic Nephropathy Rat Model |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPC123Hu01 | Recombinant SMAD family member 3 (SMAD3) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC123Hu01 | Polyclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| FAC123Hu01 | Anti-SMAD family member 3 (SMAD3) Polyclonal Antibody | Flow cytometry. |
| MAC123Hu21 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu22 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |
| MAC123Hu23 | Monoclonal Antibody to SMAD family member 3 (SMAD3) | WB; IHC; ICC; IP. |