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Human U2OS cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-huxxx mouse Polyclonal Antibody (Catalog FAB810Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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Anti-Vitamin D Binding Protein (DBP) Polyclonal Antibody
GC; VDBG; VDBP; Group-Specific Component; Gc-globulin
- Product No.FAB810Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Potency (Clone Number)n/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Excitation Laser
- Vol. per test
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Organism Species Moren/a
- ApplicationsFlow cytometry.
- Downloadn/a
- UOM 25test50test 100test 200test 500test
- FOB
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For more details, please contact local distributors!
USAGE
Flow cytometry: 20µg/ml;
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
GIVEAWAYS
INCREMENT SERVICES
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Protein A/G Purification Column
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Positive Control for Antibody
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Tissue/Sections Customized Service
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Phosphorylated Antibody Customized Service
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Flow Cytometry (FCM) Experiment Service
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Immunoprecipitation (IP) Experiment Service
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Immunofluorescence (IF) Experiment Service
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Buffer
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DAB Chromogen Kit
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SABC Kit
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Isotype Control
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Real Time PCR Experimental Service
| Magazine | Citations |
| Proteome Science | Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients PubMed: 23101585 |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients Pubmed:25590278 |
| Endocrine | Vitamin D-binding protein and free vitamin D concentrations in acromegaly PubMed: 26547217 |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients Pubmed:26680509 |
| PLoS One. | 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients. pubmed:28085957 |
| PROTEOMICS - Clinical Applications | Comparative mass spectrometric and immunoassay-based proteome analysis in serum ofDuchenne muscular dystrophy patients. pubmed:26680509 |
| Plos One | 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients 10.1371/journal.pone.0170323 |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … Pubmed:30037068 |
| BBA- Molecular Basis of Disease | Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic … Pubmed: 30553018 |
| J Clin Res Pediatr Endocrinol | Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children? 34013709 |
| PLoS One | Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism Pubmed:35202418 |