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Eukaryotic Ciliary Neurotrophic Factor (CNTF)
HCNTF
- Product No.EPA021Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourceEukaryotic expression
- Host293F Cell
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted, Cytoplasm
- Predicted Molecular Mass24.32kDa
- Accurate Molecular Mass25kDa(Analysis of differences refer to the manual)
- Residues & TagsAla2~Met200 with N-terminal His Tag
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point6.0
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Applications
Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein. - Downloadn/a
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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For more details, please contact local distributors!
SEQUENCE
Ciliary Neurotrophic Factor (CNTF) is a 22 kDa cytokine initially identified for its role in supporting parasympathetic neuron survival. It signals through a tripartite receptor complex to activate JAK-STAT pathways, particularly STAT3. Primarily expressed in Schwann cells and astrocytes, CNTF promotes neuronal survival, differentiation, and regeneration in both CNS and PNS. It demonstrates neuroprotective effects in models of ALS, Huntington's disease, and retinal degeneration. Unlike classical cytokines, CNTF lacks a signal peptide and is released after cellular injury. Current research explores its therapeutic potential for neurodegenerative disorders and metabolic regulation.Besides,Erythropoietin (EPO) has been identified as an interactor of CNTF, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat CNTF and recombinant human EPO. Briefly, CNTF was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to EPO-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CNTF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat CNTF and recombinant human EPO was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE
Reconstitute in PBS or others.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Spike RBD Protein (S-RBD)
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Protein G
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Protein A
| Magazine | Citations |
| Stem?Cells.? | Ciliary Neurotrophic Factor Promotes the Activation of Corneal Epithelial Stem/Progenitor Cells and Accelerates Corneal Epithelial Wound Healing Pubmed:25546438 |
| Frontiers in Molecular Neuroscience | Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis. pubmed:28959186 |
| EXPERIMENTAL EYE RESEARCH | Mesenchymal stem cells provide paracrine neuroprotective resources that delay degeneration of co-cultured organotypic neuroretinal cultures Pubmed: 31108056 |