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Active Homeobox Protein D12 (HOXD12)

HOX4H

ACTIVITY TEST

HoxD12 (homeobox D12) is a 275 amino acid protein that localizes to the nucleus and contains one homeobox DNA-binding domain. It plays a key role in embryonic limb development,which precisely controls the morphogenesis and pattern formation of bones and soft tissues in different parts of the limb. And it also can regulate the processes of cell differentiation and proliferation, during the differentiation of stem cells in certain tissues, the HOXD12 protein can guide cells to differentiate in a specific direction.It is reported that Hoxd-12 can bind to and transactivate the Shh promoter in vitro.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human HOXD12 and recombinant human Shh. Briefly, biotin-linked HOXD12 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to Shh-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of HOXD12 and Shh was shown in Figure 1, the EC50 for this effect is 0.05ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APP949Hu01 Active Homeobox Protein D12 (HOXD12) Cell culture; Activity Assays.
RPP949Hu01 Recombinant Homeobox Protein D12 (HOXD12) Positive Control; Immunogen; SDS-PAGE; WB.