Packages (Simulation)
Image (I)
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Figure . Gene Sequencing (extract)
Image (II)
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Figure. SDS-PAGE
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WB Image
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Instruction Manual
Certificate
Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
FRCP2; Irisin; Fibronectin type III repeat-containing protein 2
- Product No.APN576Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point5.0
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 270
US$ 675
US$ 1350
US$ 4050
US$ 10125
For more details, please contact local distributors!
ACTIVITY TEST
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. FNDC5 is a 12 kDa glycosylated polypeptide hormone that regulate glycolipid metabolism, cardiovascular homeostasis metabolism, stem cell differentiation, and neuronal development. Brain Derived Neurotrophic Factor (BDNF) has been identified as an interactor of FNDC5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FNDC5 and recombinant dog BDNF. Briefly, FNDC5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to BDNF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FNDC5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human FNDC5 and recombinant dog BDNF was shown in Figure 1, the EC50 for this effect is 1.64 ug/mL.
USAGE
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Diabetes Research and Clinical Practice | Serum irisin levels in new-onset type 2 diabetes PubMed: 23369227 |
| Journal of diabetes and its complications | Lower circulating irisin is associated with type 2 diabetes mellitus Pubmed: 23619195 |
| Horm Metab Res. | Inconsistency in circulating irisin levels: what is really happening Pubmed:24459033 |
| Int J Obes (Lond). | Leptin administration activates irisin-induced myogenesis via nitric oxide-dependent mechanisms, but reduces its effect on subcutaneous fat browning in mice Pubmed:25199621 |
| Molecular and Cellular Endocrinology | Fenofibrate (PPARalpha agonist) induces beige cell formation in subcutaneous white adipose tissue from diet-induced male obese mice Pubmed:25576856 |
| journal of diabetes | Lower irisin level in patients with type 2 diabetes mellitus: A case-control study and meta-analysis PubMed: 25494632 |
| The Journal of Maternal-Fetal & Neonatal Medicine | Maternal circulating levels of irisin in intrahepatic cholestasis of pregnancy PubMed: 26689349 |
| Hormone and Metabolic Research | Lower Circulating Irisin Level in Patients with Diabetes Mellitus: A Systematic Review and Meta-Analysis Pubmed:27300472 |
| Nature Reviews Endocrinology | Physiology and role of irisin in glucose homeostasis. pubmed:28211512 |
| Journal of Molecular and Cellular Cardiology | Irisin improves perivascular adipose tissue dysfunction via regulation of the heme oxygenase-1/adiponectin axis in diet-induced obese mice pubmed:27638193 |
| Biochemical and Biophysical Research Communications | Irisin stimulates cell proliferation and invasion by targeting the PI3K/AKT pathway in human hepatocellular carcinoma pubmed:28867187 |
| PLoS One | Differential actions of PPAR-α and PPAR-β/δ on beige adipocyte formation: A study in the subcutaneous white adipose tissue of obese male mice Pubmed:29351550 |
| International Brazilian Journal of Urology | The Diagnostic Value of FNDC5/Irisin in Renal Cell Cancer. Pubmed:29522296 |
| Redox Biology | Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative … Doi: 10.1016/j.redox.2018.10.019 |
| Antioxidants & redox signaling | Low serum irisin concentration is associated with poor outcomes in patients with acute pancreatitis and irisin administration protects against experimental acute … Pubmed: 31250660 |
| Journal of Maternal-Fetal & Neonatal Medicine | Maternal serum, placental, and umbilical venous blood irisin levels in intrahepatic cholestasis of pregnancy Pubmed: 31590596 |
| Biomed Pharmacother | Identification of irisin as a therapeutic agent that inhibits oxidative stress and fibrosis in a murine model of chronic pancreatitis Pubmed: 32199226 |
| Irisin Improves Autophagy of Aged Hepatocytes via Increasing Telomerase Activity in Liver Injury Pubmed: 31976032 | |
| Serum Irisin Predicts Posthepatectomy Complications in Patients with Hepatocellular Carcinoma Pubmed: 31976024 | |
| DOMESTIC ANIMAL ENDOCRINOLOGY | The myokine Irisin: localization and effects in swine late medium and large antral ovarian follicle Pubmed: 33120167 |
| Journal of Biomaterials and Tissue Engineering | The Combination of Aerobic and Resistance Exercise Induces Weight Loss via the PGC-1α/Irisin/UCP-1 Pathway |
| Evaluation of a recombinant bacillus calmette-guérin vaccine expressing P39-L7/L12 of Brucella melitensis: An immunization strategy against brucellosis in BALB/c … | |
| Eur Rev Med Pharmacol Sci | Salivary Irisin: potential inflammatory biomarker in recurrent apthous stomatitis patients 33755963 |
| Metabolites | Pattern of Adiponectin, Osteocalcin, Irisin, FGF-21, and MCP-1 According to the Body Size Phenotype: Could They Be Markers of Metabolic Health in Mexican-Mestizo … 34822430 |
| Eat Weight Disord | Time-restricted feeding prevents metabolic diseases through the regulation of galanin/GALR1 expression in the hypothalamus of mice 34370270 |
| Ir J Med Sci | Eight-week exercise program improved the quality of life of Alzheimer's patients through functional, cognitive, and biochemical parameters Pubmed:35604540 |
| Biomed Pharmacother | Irisin attenuates sepsis-induced cardiac dysfunction by attenuating inflammation-induced pyroptosis through a mitochondrial ubiquitin ligase-dependent … Pubmed:35653888 |
| Journal of King Saud University - Science | Interaction of nitric oxide synthase and mitochondrial ATP-sensitive potassium channels in protective impacts of combination therapy with irisin … |