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Active Protein L-Isoaspartate-O-Methyltransferase (PCMT1)

PIMT; L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl/D-aspartyl methyltransferase; Protein-beta-aspartate methyltransferase

ACTIVITY TEST

Protein L-Isoaspartate-O-Methyltransferase (PCMT1) is a crucial enzyme involved in the repair of damaged proteins. It catalyzes the methylation of abnormal L-isoaspartyl residues and, to a lesser extent, D-aspartyl residues in proteins, which arise from the spontaneous deamidation and isomerization of asparagine and aspartate residues over time. This post-translational modification serves as a key step in the repair process, allowing these damaged proteins to be either restored to their normal structure or targeted for degradation, thereby maintaining protein homeostasis within cells. As PCMT1 has the function of cell proliferation , we measure the activity of recombinant human PCMT1 by the ability of the protein to stimulate MCF-7 cells proliferation. MCF-7 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human PCMT1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human PCMT1 for 72h. The result was shown in Figure 1. It was obvious that PCMT1 significantly increased cell viability of MCF-7 cells. The ED50 of recombinant human PCMT1 is 2.059 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPL997Hu01 Recombinant Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Positive Control; Immunogen; SDS-PAGE; WB.
APL997Hu01 Active Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Cell culture; Activity Assays.
PAL997Hu01 Polyclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) WB; IHC
MAL997Hu21 Monoclonal Antibody to Protein L-Isoaspartate-O-Methyltransferase (PCMT1) WB; IHC; ICC; IP.
SEL997Hu ELISA Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) Enzyme-linked immunosorbent assay for Antigen Detection.
LML997Hu Multiplex Assay Kit for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PGL997Hu01 Primer Pair for Protein L-Isoaspartate-O-Methyltransferase (PCMT1) PCR