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Active Interleukin 1 Family, Member 9 (IL1F9)

IL-1F9; IL36g; IL-1H1; IL-1RP2; IL1E; IL1H1; IL1RP2; Interleukin 36, Gamma; Interleukin-1 Homolog 1; Interleukin-1 epsilon; Interleukin 1-Related Protein 2

ACTIVITY TEST

IL36G (Interleukin 36 gamma), also known as IL-1F9 and IL-1H1 is a member of the IL-1 family of cytokines. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. It has been reported that IL-1F9 can induced IL-8 production in HacaT cells. To test the bioactivity of IL-1F9, HacaT cells were seeded into 24-well plate at a density of 1x105 cells/mL , and allowed to attach overnight before treated with certain concentrations of IL-1F9 for 24h and IL-8 levels in the cell supernatant were determined by ELISA. IL-8 levels in the cell supernatant of HacaT cells increased significantly after stimulated with IL-1F9 have shown in Figure1.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
PLOS ONE Interleukin (IL)-1β Is a Strong Inducer of IL-36γ Expression in Human Colonic Myofibroblasts PubMed: 26562662
Journal of Investigative Dermatology Increased interleukin‐36γ expression in skin and sera of patients with atopic dermatitis and mycosis fungoides/Sézary syndrome Pubmed:29314201
Journal of Investigative Dermatology For Review Only
international immunopharmacology Acitretin inhibits IL-17A-induced IL-36 expression in keratinocytes by down-regulating IκBζ Pubmed: 31863918
J Agric Food Chem Inhibition of HMGB1/TLR4 Signaling Pathway by Digitoflavone: A Potential Therapeutic Role in Alcohol-Associated Liver Disease Pubmed:35212223
Br J Pharmacol Modulation of IL‐36‐based inflammatory feedback loop through hepatocytes‐derived IL‐36R‐P2X7R axis improves steatosis in alcoholic steatohepatitis Pubmed:35481896
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPL621Ra01 Recombinant Interleukin 1 Family, Member 9 (IL1F9) Positive Control; Immunogen; SDS-PAGE; WB.
APL621Ra01 Active Interleukin 1 Family, Member 9 (IL1F9) Cell culture; Activity Assays.
PAL621Ra01 Polyclonal Antibody to Interleukin 1 Family, Member 9 (IL1F9) WB; IHC; ICC; IP.