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Active Filaggrin (FLG)

Profilaggrin

ACTIVITY TEST

Filaggrin (FLG), a key keratinocyte differentiation protein encoded by the FLG gene, is critical for skin barrier formation. Synthesized as a large precursor, profilaggrin, it is cleaved into functional filaggrin monomers in the stratum granulosum of the epidermis. These monomers bind to keratin filaments, promoting their aggregation to form a dense, protective cytoskeletal structure. Filaggrin also contributes to skin hydration by breaking down into natural moisturizing factors. FLG interacts with SPINK5, which encodes the serine protease inhibitor LEKTI; SPINK5 regulates proteases that process profilaggrin into filaggrin, maintaining proper barrier function.Briefly, SPINK5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to FLG-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SPINK5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately.Measured by its binding ability in a functional ELISA. When Recombinant FLG is lmmobilized at 2 µg/mL(100 uLwell), the concentration of SPINK5 that produces 50% optimal bindingresponse is found to be approximately11.830 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of the Egyptian Women's Dermatologic Society Filaggrin R501x and 2282del4 gene mutations and its tissue levels in patients with chronic plaque psoriasis LWW: 01000
?Inflammation & Allergy-Drug Targets Neuromodulatory and Anti-Inflammatory Ingredient for Sensitive Skin: In Vitro Assessment Pubmed:24931812
PLoS One Protective Effects of Triphala on Dermal Fibroblasts and Human Keratinocytes Pubmed:26731545
Journal of food biochemistry In vitro anti-inflammatory and skin protective properties of extract comprising ceramides from de-oiled rice bran doi:10.1111
European Journal of Pharmacology Imiquimod-induced psoriasis-like inflammation in differentiated Human keratinocytes: Its evaluation using curcumin. pubmed:28736282
Journal of Dermatological Science A novel, topical, nonsteroidal, TRPV1 antagonist, PAC-14028 cream improves skin barrier function and exerts anti-inflammatory action through modulating epidermal … Pubmed:29752146
Journal of Traditional and Complementary Medicine In vitro anti-inflammatory and skin protective properties of Virgin coconut oil 10.1016:j.jtcme.2017.06.012
Clinical and Molecular Allergy Markers of atopic dermatitis, allergic rhinitis and bronchial asthma in pediatric patients: correlation with filaggrin, eosinophil major basic protein and …
Intractable & Rare Diseases Research Filaggrin, major basic protein and leukotriene B4: Biomarkers for adult patients of bronchial asthma, atopic dermatitis and allergic rhinitis Pubmed: 30560019
Dermatology and Therapy Epidermal and Dermal Hallmarks of Photoaging are Prevented by Treatment with Night Serum Containing Melatonin, Bakuchiol, and Ascorbyl …
日本化粧品技術者会誌 糖化に起因する表皮機能低下に対するワイルドタイムエキスの防御効果
J Dermatol Filaggrin‐gene mutation has minimal effect on the disease severity in the lesions of atopic dermatitis 34322929
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPJ103Hu02 Recombinant Filaggrin (FLG) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ103Hu01 Recombinant Filaggrin (FLG) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ103Hu05 Recombinant Filaggrin (FLG) Positive Control; Immunogen; SDS-PAGE; WB.
EPJ103Hu61 Eukaryotic Filaggrin (FLG) Positive Control; Immunogen; SDS-PAGE; WB.
RPJ103Hu03 Recombinant Filaggrin (FLG) Positive Control; Immunogen; SDS-PAGE; WB.
APJ103Hu61 Active Filaggrin (FLG) Cell culture; Activity Assays.
RPJ103Hu04 Recombinant Filaggrin (FLG) Positive Control; Immunogen; SDS-PAGE; WB.
PAJ103Hu01 Polyclonal Antibody to Filaggrin (FLG) WB
PAJ103Hu02 Polyclonal Antibody to Filaggrin (FLG) Testing in progress
LAJ103Hu71 Biotin-Linked Polyclonal Antibody to Filaggrin (FLG) WB; IHC; ICC.
LAJ103Hu83 FITC-Linked Polyclonal Antibody to Filaggrin (FLG) WB; IHC; ICC; IF.
LAJ103Hu81 FITC-Linked Polyclonal Antibody to Filaggrin (FLG) WB; IHC; ICC; IF.
MAJ103Hu21 Monoclonal Antibody to Filaggrin (FLG) Testing in progress
SEJ103Hu ELISA Kit for Filaggrin (FLG) Enzyme-linked immunosorbent assay for Antigen Detection.
SCJ103Hu CLIA Kit for Filaggrin (FLG) Chemiluminescent immunoassay for Antigen Detection.
LMJ103Hu Multiplex Assay Kit for Filaggrin (FLG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.