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Active Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1)

GPIPLD; GPIPLDM; PIGPLD; PIGPLD1; Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D

ACTIVITY TEST

Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) is a secreted enzyme that cleaves glycosylphosphatidylinositol (GPI) anchors, releasing GPI-anchored proteins from cell membranes into circulation. Primarily synthesized in the liver, it plays key roles in lipid metabolism, inflammation, and neurogenesis. GPLD1's activity modulates the bioavailability of important GPI-anchored proteins like alkaline phosphatase and folate receptors. Studies link elevated GPLD1 levels to metabolic disorders and aging-related cognitive decline, while its neuroprotective effects are being investigated. The enzyme's structure contains catalytic domains essential for hydrolyzing GPI linkages, making it a potential therapeutic target for metabolic and neurological diseases.Besides,Alkaline Phosphatase, Tissue-nonspecific (ALPL) has been identified as an interactor of GPLD1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GPLD1 and recombinant human ALPL. Briefly, GPLD1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ALPL-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-GPLD1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human GPLD1 and recombinant human ALPL was shown in Figure 1, the EC50 for this effect is 0.042µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
International Journal of Clinical and Experimental Medicine Int J Clin Exp Med. 2012; 5(4): 306–309. Plasma glycosylphosphatidylinositol phospholipase D (GPI-PLD) and abdominal aortic aneurysm PubMed: PMC3443889
J Endocrinol Invest Serum glypican4 and glycosylphosphatidylinositol-specific phospholipase D levels are associated with adipose tissue insulin resistance in obese subjects with … Pubmed: 32816247
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPH975Hu01 Recombinant Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) Positive Control; Immunogen; SDS-PAGE; WB.
APH975Hu01 Active Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) Cell culture; Activity Assays.
PAH975Hu01 Polyclonal Antibody to Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) IHC
MAH975Hu21 Monoclonal Antibody to Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) WB; IHC; ICC; IP.
SEH975Hu ELISA Kit for Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH975Hu Multiplex Assay Kit for Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.