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Active Homeobox Protein B4 (HOXB4)

HOX2; HOX-2.6; HOX2F

ACTIVITY TEST

HOXB4 protein is a transcription factor encoded by the HOXB4 gene within the HOXB gene cluster of the homeobox gene family.During embryonic development, HOXB4 plays a pivotal role in anterior - posterior (A - P) axis patterning. In hematopoiesis, HOXB4 is a key regulator. It promotes the self - renewal of hematopoietic stem cells (HSCs). Besides,Sex Determining Region Y Box Protein 2 (SOX2) has been identified as an interactor of HOXB4, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human HOXB4 and recombinant human SOX2 . Briefly, biotin-linked HOXB4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to SOX2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of HOXB4 and SOX2 was shown in Figure 1, the EC50 for this effect is 0.044ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPH890Hu01 Recombinant Homeobox Protein B4 (HOXB4) Positive Control; Immunogen; SDS-PAGE; WB.
APH890Hu01 Active Homeobox Protein B4 (HOXB4) Cell culture; Activity Assays.