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Active Osteocrin (OSTN)

Musclin

ACTIVITY TEST

Osteocrin (OSTN) is a secreted protein predominantly expressed in bone and muscle tissues. As an endogenous ligand for natriuretic peptide receptors, it plays a crucial role in regulating bone growth, metabolism, and musculoskeletal development. Structurally, OSTN shares similarities with natriuretic peptides and modulates cardiovascular and skeletal homeostasis through competitive binding. Notably, it binds with high affinity to Natriuretic Peptide Receptor 3 (NPR3). This binding interaction inhibits the degradation of other natriuretic peptides, thereby enhancing their bioavailability and prolonging their physiological effects on blood pressure regulation and cardiovascular protection.To detect the activity of recombinant OSTN a functional ELISA assay was performed to evaluate the interaction between recombinant rat OSTN and recombinant rat NPR3.Briefly, OSTN was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NPR3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-OSTN pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat OSTN and recombinant rat NPR3 was shown in Figure 1, the EC50 for this effect is 0.10 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Acta Biomater Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study PubMed: 26689470
Acta Biomaterialia Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study Pubmed:26689470
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
EPH492Ra61 Eukaryotic Osteocrin (OSTN) Positive Control; Immunogen; SDS-PAGE; WB.
APH492Ra61 Active Osteocrin (OSTN) Cell culture; Activity Assays.
RPH492Ra01 Recombinant Osteocrin (OSTN) Positive Control; Immunogen; SDS-PAGE; WB.
PAH492Ra01 Polyclonal Antibody to Osteocrin (OSTN) WB; IHC; ICC; IP.
MAH492Ra21 Monoclonal Antibody to Osteocrin (OSTN) Testing in progress
SEH492Ra ELISA Kit for Osteocrin (OSTN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH492Ra Multiplex Assay Kit for Osteocrin (OSTN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.