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Active UDP Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1)

UGT1; GNT1; UGT1A; UDPGT; HUG-BR1; UGT1-1; Bilirubin-specific UDPGT isozyme 1; UDP-glucuronosyltransferase 1-1

ACTIVITY TEST

UDP-Glucuronosyltransferase 1 Family, Polypeptide A1 (UGT1A1)，as known as GNT1 or UGT1，is a member of the UDP-glucuronosyltransferase (UGT) enzyme family, which plays a critical role in the metabolism of various compounds in the body. This enzyme catalyzes the conjugation of bilirubin, steroids, and other lipophilic compounds with glucuronic acid, facilitating their excretion from the body.The binding of UGT1A1 and Cytochrome P450 1A1 (CYP1A1) is particularly importantin the metabolism of drugs and xenobiotics, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human UGT1A1 and recombinant rat CYP1A1. Briefly, biotin-linked UGT1A1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to CYP1A1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of UGT1A1 and CYP1A1 was shown in Figure 1, the EC50 for this effect is 0.08ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats Pubmed:25733458
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,5 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
Journal of Medicinal Chemistry A practical and high-affinity fluorescent probe for uridine diphosphate glucuronosyltransferase 1A1: a good surrogate for bilirubin pubmed:29125289
International Journal of Nanomedicine Gold Nanoparticles Perturb Drug-Metabolizing Enzymes and Antioxidants in the Livers of Male Rats: Potential Impact on Drug Interactions Pubmed: 32764932
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