≡

Active Secreted Frizzled Related Protein 2 (SFRP2)

FRP-2; SARP1; SDF-5; Secreted apoptosis-related protein 1

ACTIVITY TEST

Secreted Frizzled Related Protein 2 (SFRP2) is a key modulator of the Wnt signaling pathway. It harbors a frizzled-like cysteine-rich domain (CRD) that shares homology with the Wnt-binding region of Frizzled receptors, and a C-terminal netrin domain. As a core member of the SFRP family, SFRP2 localizes in the extracellular space and exerts dual regulatory effects on Wnt signaling: it acts as an antagonist of canonical Wnt signaling while activating the noncanonical Wnt/Ca²⁺ pathway, participating in embryonic development, angiogenesis, tissue regeneration and tumorigenesis. It is widely expressed in the alimentary system, brain, embryo mesenchyme and other tissues, and its abnormal expression is associated with cardiovascular diseases and various cancers. SFRP2 binds directly to WNT2 via its CRD domain, blocking WNT2's interaction with Frizzled receptors to inhibit canonical Wnt signaling.Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat SFRP2 and recombinant human WNT2. Briefly, SFRP2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to WNT2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SFRP2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat SFRP2 and recombinant human WNT2 was shown in Figure 1, the EC50 for this effect is 0.181 µg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Experimental & Clinical Cancer Research PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway pubmed:28645312
Biochemical genetics Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer pubmed:28421310
Respiratory physiology & neurobiology sFRP2 promotes airway inflammation and Th17/Treg imbalance in COPD via Wnt/β-catenin pathway Pubmed: 31430541
Stem Cell Research Ocular Surface Ectoderm Instigated by WNT inhibition and BMP4 Pubmed: 32603880
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APF879Ra01 Active Secreted Frizzled Related Protein 2 (SFRP2) Cell culture; Activity Assays.
RPF879Ra01 Recombinant Secreted Frizzled Related Protein 2 (SFRP2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF879Ra01 Polyclonal Antibody to Secreted Frizzled Related Protein 2 (SFRP2) WB; IHC; ICC; IP.
MAF879Ra21 Monoclonal Antibody to Secreted Frizzled Related Protein 2 (SFRP2) WB; IHC; ICC; IP.
SEF879Ra ELISA Kit for Secreted Frizzled Related Protein 2 (SFRP2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF879Ra Multiplex Assay Kit for Secreted Frizzled Related Protein 2 (SFRP2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.