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Active Secreted Frizzled Related Protein 2 (SFRP2)

FRP-2; SARP1; SDF-5; Secreted apoptosis-related protein 1

ACTIVITY TEST

Secreted Frizzled-Related Protein 2 (SFRP2) is a key member of the SFRP family of extracellular signaling molecules. By inhibiting the Wnt/β-catenin pathway, SFRP2 plays a crucial role in regulating fundamental cellular processes. Its primary functions include the suppression of cellular proliferation and the induction of apoptosis. To test the effect of SFRP2 on cell proliferation, A549 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human SFRP2. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human SFRP2 for 72h. The result was shown in Figure 1. It was obvious that SFRP2 significantly increased cell viability of A549 cells. The ED50 of recombinant human SFRP2 is 1.655 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Experimental & Clinical Cancer Research PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway pubmed:28645312
Biochemical genetics Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer pubmed:28421310
Respiratory physiology & neurobiology sFRP2 promotes airway inflammation and Th17/Treg imbalance in COPD via Wnt/β-catenin pathway Pubmed: 31430541
Stem Cell Research Ocular Surface Ectoderm Instigated by WNT inhibition and BMP4 Pubmed: 32603880
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