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Active N-Myristoyltransferase 1 (NMT1)

Myristoyl-CoA:protein N-myristoyltransferase 1; Glycylpeptide N-tetradecanoyltransferase 1

ACTIVITY TEST

N-Myristoyltransferase 1 (NMT1) is an indispensable eukaryotic enzyme that belongs to the NMT family. NMT1 can catalyses the transfer of myristoyl groups to the amino acid terminal residues of numerous proteins. This catalytic process is required for the growth and development of many eukaryotes and viruses. Furthermore, it is recognized that N-myristoylation can also occur posttranslationally on an internal glycine exposed by caspase cleavage during apoptosis.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human NMT1 and recombinant human CASP3. Briefly, biotin-linked NMT1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to CASP3-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of human NMT1 and human CASP3 was shown in Figure 1, the EC50 for this effect is 0.01384ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPF673Hu01 Recombinant N-Myristoyltransferase 1 (NMT1) Positive Control; Immunogen; SDS-PAGE; WB.
APF673Hu01 Active N-Myristoyltransferase 1 (NMT1) Cell culture; Activity Assays.