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Active Mitofusin 2 (MFN2)

CMT2A; CMT2A2; CPRP1; HSG; MARF; Transmembrane GTPase MFN2

ACTIVITY TEST

Mitofusin 2 (MFN2) is a dynamin-like GTPase protein primarily located on the outer membrane of mitochondria. It is a master regulator of mitochondrial dynamics, mediating the fusion of mitochondria to maintain their shape, distribution, and functional health. This process is crucial for cellular energy metabolism, calcium signaling, and overall stress response. MFN2 is also uniquely present on the endoplasmic reticulum (ER), where it plays a pivotal role in forming physical tethers between the ER and mitochondria, establishing specialized domains known as Mitochondria-Associated Membranes (MAMs). These contact sites are essential for lipid transfer, apoptotic signaling, and coordinated calcium flux. MFN2 directly binds to VDAC1 on the mitochondrial surface to bridge the ER-mitochondrial interface, facilitating inter-organelle communication.To detect the activity of recombinant MFN2, a functional ELISA assay was performed to evaluate the interaction between recombinant rat MFN2 and recombinant human VDAC1.Briefly, biotin-linked MFN2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to VDAC1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of MFN2 and VDAC1 was shown in Figure 1, the EC50 for this effect is 0.12µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J Clin Med Relationship between Cardiopulmonary, Mitochondrial and Autonomic Nervous System Function Improvement after an Individualised Activity Programme upon?¡­ 33917586
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APF591Ra01 Active Mitofusin 2 (MFN2) Cell culture; Activity Assays.
RPF591Ra01 Recombinant Mitofusin 2 (MFN2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF591Ra01 Polyclonal Antibody to Mitofusin 2 (MFN2) WB
SEF591Ra ELISA Kit for Mitofusin 2 (MFN2) Enzyme-linked immunosorbent assay for Antigen Detection.
CMF591Ra Multiplex Assay Kit for Mitofusin 2 (MFN2) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.
LMF591Ra Multiplex Assay Kit for Mitofusin 2 (MFN2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.