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Active Centaurin Alpha 2 (CENTa2)

ADAP2; Cent-b; Arf-GAP With Dual PH Domain-Containing Protein 2

ACTIVITY TEST

Centaurin Alpha 2 (CENTa2) is a member of the Centaurin family of proteins, which are characterized by their unique structure and function in intracellular signaling and endosomal trafficking. It plays a role in various cellular processes by interacting with other molecules and is implicated in the regulation of cell growth, migration, and differentiation.
Besides,Neurofibromin 1 (NF1) has been identified as an interactor of CENTa2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CENTa2 and recombinant human NF1. Briefly, CENTa2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NF1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CENTa2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse CENTa2 and recombinant human NF1 was shown in Figure 1, the EC50 for this effect is 2.96ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
APF466Mu01 Active Centaurin Alpha 2 (CENTa2) Cell culture; Activity Assays.
RPF466Mu01 Recombinant Centaurin Alpha 2 (CENTa2) Positive Control; Immunogen; SDS-PAGE; WB.
PAF466Mu01 Polyclonal Antibody to Centaurin Alpha 2 (CENTa2) WB; IHC; ICC; IP.