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Active Interleukin 1 Zeta (IL1z)

IL1F7; IL37; FIL1; FIL1(ZETA); FIL1Z; IL-1F7; IL-1H4; IL-1RP1; IL1H4; IL1RP1; Interleukin 37; Interleukin-1-Related Protein; Interleukin 1 Family, Member 7

ACTIVITY TEST

Interleukin 37, also known as Interleukin 1 Zeta (IL1z), is an anti-inflammatory cytokine. Interleukines are cytokines that make an important part of immune signaling. It belongs to the interleukin-1 family. IL-37 plays a role in protecting the body against endotoxin shock, ischemia-reperfusion injury, autoimmune diseases, and cardiovascular diseases. In addition, IL-37 has a potential antitumor effect. Besides, Interleukin 18 Receptor 1 (IL18R1) has been identified as an interactor of IL1z, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL1z and recombinant human IL18R1. Briefly, IL1z were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to IL18R1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IL1z pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of IL1z and IL18R1 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Journal of Periodontal Research Levels of interleukin-37 in gingival crevicular fluid, saliva, or plasma in periodontal disease Pubmed:25399716
Arthritis research & therapy Interleukin 37 limits monosodium urate crystal-induced innate immune responses in human and murine models of gout. pubmed:27863506
Thoracic Cancer  IL‐37 inhibits invasion and metastasis in non‐small cell lung cancer by suppressing the IL‐6/STAT3 signaling pathway Pubmed:29575809
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APE842Hu01 Active Interleukin 1 Zeta (IL1z) Cell culture; Activity Assays.
RPE842Hu01 Recombinant Interleukin 1 Zeta (IL1z) Positive Control; Immunogen; SDS-PAGE; WB.
RPE842Hu02 Recombinant Interleukin 1 Zeta (IL1z) Positive Control; Immunogen; SDS-PAGE; WB.
APE842Hu02 Active Interleukin 1 Zeta (IL1z) Cell culture; Activity Assays.
PAE842Hu01 Polyclonal Antibody to Interleukin 1 Zeta (IL1z) WB; IHC; ICC; IP.
PAE842Hu02 Polyclonal Antibody to Interleukin 1 Zeta (IL1z) WB; IHC; ICC; IP.
LAE842Hu81 FITC-Linked Polyclonal Antibody to Interleukin 1 Zeta (IL1z) WB; IHC; ICC; IF.
LAE842Hu71 Biotin-Linked Polyclonal Antibody to Interleukin 1 Zeta (IL1z) WB; IHC; ICC.
MAE842Hu22 Monoclonal Antibody to Interleukin 1 Zeta (IL1z) WB; IHC; ICC; IP.
SEE842Hu ELISA Kit for Interleukin 1 Zeta (IL1z) Enzyme-linked immunosorbent assay for Antigen Detection.
SCE842Hu CLIA Kit for Interleukin 1 Zeta (IL1z) Chemiluminescent immunoassay for Antigen Detection.
LME842Hu Multiplex Assay Kit for Interleukin 1 Zeta (IL1z) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.