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Active Xeroderma Pigmentosum, Complementation Group B (XPB)

ERCC3; XPBC; BTF2; RAD25; TFIIH; GTF2H; Excision Repair Cross-Complementing Rodent Repair Deficiency,Complementation Group 3; Basic transcription factor 2 89 kDa subunit

ACTIVITY TEST

Xeroderma Pigmentosum, Complementation Group B (XPB) is a crucial DNA helicase encoded by the ERCC3 gene in humans. As a component of the transcription factor IIH (TFIIH) complex, XPB plays dual roles in nucleotide excision repair (NER) and transcription initiation. In NER, it unwinds DNA around lesion sites to facilitate damage removal, while in transcription, it helps open promoter regions for RNA polymerase II. Besides,Poly U Binding Splicing Factor 60kDa (PUF60) has been identified as an interactor of XPB, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human XPB and recombinant human PUF60 .Briefly, biotin-linked XPB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to PUF60-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of recombinant human XPB and recombinant human PUF60 was shown in Figure 1, the EC50 for this effect is 0.29 µg/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
APE732Hu01 Active Xeroderma Pigmentosum, Complementation Group B (XPB) Cell culture; Activity Assays.
RPE732Hu01 Recombinant Xeroderma Pigmentosum, Complementation Group B (XPB) Positive Control; Immunogen; SDS-PAGE; WB.