≡

Active Lemur Tyrosine Kinase 3 (LMTK3)

LMR3; TYKLM3; Serine/threonine-protein kinase LMTK3

ACTIVITY TEST

Lemur tyrosine kinase 3 (LMTK3), a member of the receptor tyrosine kinase (RTK) family, is implicated in breast cancer growth and endocrine resistance. It has been reported that LMTK3 promotes cell invasion, motility, and migration of the MCF-7 breast cancer cell line. To test the bioactivity of LMTK3, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of LMTK3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C.
Cell proliferation of MCF-7 cells after incubation with LMTK3 for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of LMTK3 was shown in Figure 2. It was obvious that LMTK3 significantly promoted cell proliferation of MCF-7 cells. The ED50 for this effect is approximately 6.14~50ng/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Medical Oncology Serum lemur tyrosine kinase 3 expression in colorectal cancer patients predicts cancer progression and prognosis Pubmed:24174317
Int J Clin Exp Pathol Serum lemur tyrosine kinase-3: a novel biomarker for screening primary non-small cell lung cancer and predicting cancer progression Pubmed:4348877
Int J Clin Exp Med Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … PubMed: 26309506
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE375Hu01 Recombinant Lemur Tyrosine Kinase 3 (LMTK3) Positive Control; Immunogen; SDS-PAGE; WB.
APE375Hu01 Active Lemur Tyrosine Kinase 3 (LMTK3) Cell culture; Activity Assays.
PAE375Hu01 Polyclonal Antibody to Lemur Tyrosine Kinase 3 (LMTK3) WB; IHC; ICC; IP.
MAE375Hu21 Monoclonal Antibody to Lemur Tyrosine Kinase 3 (LMTK3) WB; IHC; ICC; IP.
SEE375Hu ELISA Kit for Lemur Tyrosine Kinase 3 (LMTK3) Enzyme-linked immunosorbent assay for Antigen Detection.
LME375Hu Multiplex Assay Kit for Lemur Tyrosine Kinase 3 (LMTK3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.