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Active Lemur Tyrosine Kinase 3 (LMTK3)

LMR3; TYKLM3; Serine/threonine-protein kinase LMTK3

ACTIVITY TEST

Recombinant Lemur Tyrosine Kinase 3 (LMTK3), a member of the serine-threonine-tyrosine kinase family, is a critical oncogenic protein linked to multiple cancers, including breast and prostate cancer. Abnormal LMTK3 expression disrupts signaling pathways like AKT and MAPK, promoting tumor cell proliferation, invasion, and endocrine resistance in breast cancer. As a therapeutic target, structural studies reveal LMTK3’s kinase domain has a conserved fold similar to EGFR and insulin receptor kinases, with an inactive "DYG-out" conformation in its basal state. Computational modeling and molecular dynamics simulations have further characterized its structural dynamics, highlighting its role in regulating estrogen receptor-α (ERα) signaling in breast cancer. LMTK3 binds ATP via a conserved cavity with critical residues Tyr185 and Asp284, forming a stable complex essential for its kinase activity.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human LMTK3 and recombinant ATP. Briefly, LMTK3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ATP-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-LMTK3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human LMTK3 and recombinant ATP was shown in Figure 1, the EC50 for this effect is 1.76192µg/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Medical Oncology Serum lemur tyrosine kinase 3 expression in colorectal cancer patients predicts cancer progression and prognosis Pubmed:24174317
Int J Clin Exp Pathol Serum lemur tyrosine kinase-3: a novel biomarker for screening primary non-small cell lung cancer and predicting cancer progression Pubmed:4348877
Int J Clin Exp Med Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar … PubMed: 26309506
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