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Active Ephrin B2 (EFNB2)

EPLG5; HTKL; Htk-L; LERK5; HTK Ligand; Ligand Of Eph-Related Kinase 5; Eph-Related Receptor Tyrosine Kinase Ligand 5

ACTIVITY TEST

EphrinB2 (EFNB2), a 40 kDa member of the EphrinB family, is a pivotal bidirectional signaling molecule ubiquitously expressed in mammals and is crucial in angiogenesis during development and disease progression. Besides, EFNB2 is expressed at abnormally high levels in some neoplasms, such as squamous cell carcinoma of the head and neck and colorectal cancer. Its overexpression is associated with the malignant progression of tumors. Reelin (RELN) has been identified as an interactor of EFNB2, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNB2 and recombinant human RELN. Briefly, EFNB2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to RELN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-EFNB2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human EFNB2 and recombinant human RELN was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
ATS Journals Soluble Ephrin-B2 is a Novel Biomarker for Idiopathic Pulmonary Fibrosis Doi: Pdf
D50. PEDIATRIC RARE LUNG DISEASES Soluble Ephrin-B2 Is A Novel Biomarker Of Pulmonary Fibrosis doi:A7217
An International Journal of Medicine  P055 Over-expression of IL-6 and OSM impacts the polarization of pro-fibrotic macrophages and the development of bleomycin-induced lung fibrosis content:109
An International Journal of Medicine P057 Soluble Ephrin-B2 is a Prognostic Biomarker of Pulmonary Fibrosis S40.2.extract
4 ADAM10-mediated ephrin-B2 shedding promotes myofibroblast activation and organ fibrosis pubmed:29058717
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPE112Hu02 Recombinant Ephrin B2 (EFNB2) Positive Control; Immunogen; SDS-PAGE; WB.
APE112Hu02 Active Ephrin B2 (EFNB2) Cell culture; Activity Assays.
RPE112Hu01 Recombinant Ephrin B2 (EFNB2) Positive Control; Immunogen; SDS-PAGE; WB.
PAE112Hu01 Polyclonal Antibody to Ephrin B2 (EFNB2) WB; IHC; ICC; IP.
MAE112Hu21 Monoclonal Antibody to Ephrin B2 (EFNB2) WB; IHC; ICC; IP.
SEE112Hu ELISA Kit for Ephrin B2 (EFNB2) Enzyme-linked immunosorbent assay for Antigen Detection.
LME112Hu Multiplex Assay Kit for Ephrin B2 (EFNB2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.