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Active Cathepsin G (CTSG)

CG; CTS-G

ACTIVITY TEST

Cathepsin G (CTSG) is a neutral proteinase secreted by neutrophil granulocytes. It belongs to the family of serine proteases. Cathepsin G is a glycoprotein, which contains 1% carbohydrate. The activity assay of recombinant rat CTSG was measured by its ability to cleave the peptide substrate Suc-Ala-Ala Pro-Phe-pNA. The reaction was performed in 160 mM Tris-HCl, 1.6 M NaCl, pH 7.4 (Assay Buffer). The CTSG was diluted to different concentrations and the reaction was initiated by adding 50 ul CTSG to 50 ul 1 mM substrate and then read at 405 nm in kinetic mode for 5 minutes. The specific activity of recombinant rat CTSG is >70 pmol/min/µg.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
journal of maternal-fetal & neonatal medicine Cervical fluid calreticulin and cathepsin-G in pregnancies complicated by preterm prelabor rupture of membranes. pubmed:28152632
Lung Mass Spectrometry Analysis of the Exhaled Breath Condensate and Proposal of Dermcidin and S100A9 as Possible Markers for Lung Cancer Prognosis Pubmed: 31115649
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
APD993Ra01 Active Cathepsin G (CTSG) Cell culture; Activity Assays.
RPD993Ra01 Recombinant Cathepsin G (CTSG) Positive Control; Immunogen; SDS-PAGE; WB.
PAD993Ra01 Polyclonal Antibody to Cathepsin G (CTSG) WB; IHC; ICC; IP.
MAD993Ra21 Monoclonal Antibody to Cathepsin G (CTSG) WB; IHC; ICC; IP.
SED993Ra ELISA Kit for Cathepsin G (CTSG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD993Ra Multiplex Assay Kit for Cathepsin G (CTSG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.