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Active Glutamine synthetase (GS)

GLNS; GLUL; Glutamate-Ammonia Ligase; Glutamate decarboxylase

ACTIVITY TEST

Glutamine synthetase (GS) is an evolutionarily conserved, ubiquitous enzyme. It catalyzes ATP-dependent glutamate-ammonia condensation to form glutamine, a key step in nitrogen metabolism, ammonia detoxification, and nitrogen storage. With oligomeric structures and species-specific isoforms, it’s vital for animal liver/brain function, plant nitrogen assimilation, and microbial survival in nitrogen-poor environments.GS catalyzes the reaction of L-glutamate, NH₄⁺, and ATP to produce L-glutamine, ADP, and inorganic phosphate (Pi).The activity of recombinant human GS is measured by detecting the concentration of ADP generated in the reaction system using an ADP detection kit.GS perform gradient dilution to obtain concentrations of 100ug/ml, 50ug/ml, 25ug/ml, 12.5ug/ml, 6.25ug/ml, 3.13ug/ml, 1.56ug/ml, 0ug/ml.Prepare a 200 uL enzymatic reaction system according to the following components:20uL 10mM MgCl₂,40uL 20mM L-glutamate,20uL 10mM NH₄Cl, 50uL Enzyme sample. Add ATP to the system at a final concentration of 200uM ( 4uL of 100mM stock solution), mix rapidly, immediately place in a 37°C incubator, and react precisely for 30 minutes. Detect the fluorescence intensity of each well.Take the ADP standard from the ADP detection kit and perform gradient dilution to obtain concentrations of 200uM, 100uM, 50uM, 20uM, 10uM, 0uM. Plot a standard curve with the concentration of ADP standard (mM) as the x-axis and the corresponding detection value (e.g., absorbance or fluorescence intensity) as the y-axis.Using this standard curve, we calculate the corresponding concentration and subsequently determine the specific enzyme activity. The specific activity of recombinant human GS is > 100 pmol/min/ug.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
The FASEB Journal Cargo proteins of plasma astrocyte-derived exosomes in Alzheimer’s disease pubmed:27511944
Annals of Neurology  High complement levels in astrocyte‐derived exosomes of Alzheimer disease Pubmed:29406582
Journal of Neuroscience Sex differences in the glutamate signaling pathway in juvenile rats Pubmed:28861894
FASEB Journal Deficient neurotrophic factors of CSPG4-type neural cell exosomes in Alzheimer disease Pubmed:29924942
Hepatology Research Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition Pubmed: 31652385
FASEB JOURNAL Traumatic brain injury increases plasma astrocyte‐derived exosome levels of neurotoxic complement proteins Pubmed: 31916313
Translational Psychiatry Decreased mitochondrial electron transport proteins and increased complement mediators in plasma neural-derived exosomes of early psychosis Pubmed: 33106473
Drug Repurposing of Asparaginase and Vitamin C Targeting Glutamine Synthetase Improves Anticancer Effect in Metastatic Castration-resistant Prostate …
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD761Hu01 Recombinant Glutamine synthetase (GS) Positive Control; Immunogen; SDS-PAGE; WB.
APD761Hu01 Active Glutamine synthetase (GS) Cell culture; Activity Assays.
PAD761Hu01 Polyclonal Antibody to Glutamine synthetase (GS) WB; IHC
LAD761Hu71 Biotin-Linked Polyclonal Antibody to Glutamine synthetase (GS) IHC
LAD761Hu81 FITC-Linked Polyclonal Antibody to Glutamine synthetase (GS) WB; IHC; ICC; IF.
MAD761Hu21 Monoclonal Antibody to Glutamine synthetase (GS) WB; IHC; ICC; IP.
SED761Hu ELISA Kit for Glutamine synthetase (GS) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD761Hu Multiplex Assay Kit for Glutamine synthetase (GS) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
CMD761Hu Multiplex Assay Kit for Glutamine synthetase (GS) ,etc. by CBA (Cytometric Bead Array) FLIA Kit for Antigen Detection.