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Active Complement Component 1, Q Subcomponent A (C1qA)

Complement Component 1,q Subcomponent,Alpha Polypeptide

ACTIVITY TEST

Complement Component 1, Q Subcomponent A (C1qA) is a subunit of the C1q complex in the complement system. It has a crucial role in recognizing pathogens and immune complexes. Structurally, it contributes to the overall architecture of C1q, enabling it to interact with various ligands. Functionally, it is essential for initiating the classical complement pathway.Binding of C1qA to C1r activates C1r, triggering the classical complement cascade for pathogen elimination and immune response regulation.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human C1qA and recombinant mouse C1r. Briefly, C1qA was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to C1r-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-C1qA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human C1qA and recombinant mouse C1r was shown in Figure 1, the EC50 for this effect is 0.022ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
J Cell Mol Med LCN2 is a new diagnostic biomarker and potential therapeutic target in idiopathic short stature Pubmed:35610759
Journal of Biological Chemistry Competitive inhibition of the classical complement pathway using exogenous single-chain C1q recognition proteins Pubmed:35690144
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