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Active Glucosidase Alpha, Neutral AB (GaNAB)

GluII; G2AN; Alpha-glucosidase 2; Glucosidase II subunit alpha

ACTIVITY TEST

Glucosidase Alpha, Neutral AB (GaNAB) is an enzyme in the glycoside hydrolase family. It hydrolyzes alpha - glucosidic bonds, participates in carbohydrate metabolism, and is involved in endoplasmic reticulum quality control. GaNAB binds to Heat Shock 70kDa Protein 5 (HSPA5), collaborating to ensure proper glycoprotein folding and maintain cellular protein homeostasis.Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GaNAB and recombinant mouse HSPA5 .Briefly, biotin-linked GaNAB were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to HSPA5-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of GaNAB and HSPA5 was shown in Figure 1, the EC50 for this effect is 3.21ug/mL.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
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