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Active Fibrinogen Like Protein 1 (FGL1)
FGL-1; HFREP1; HP-041; LFIRE1; Hepassocin; Hepatocyte-derived fibrinogen-related protein 1; Liver fibrinogen-related protein 1
- Product No.APD022Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 95%
- Isoelectric Point5.4
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 240
US$ 600
US$ 1200
US$ 3600
US$ 9000
For more details, please contact local distributors!
ACTIVITY TEST
Fibrinogen Like Protein 1 (FGL1), also known as fibrinogen-related protein 1 or HFREP-1, is a member of the fibrinogen family. FGL1 has multiple biological functions, which plays an important role in intercellular signaling, immune regulation, cell adhesion and angiogenesis. In addition, the expression of FGL1 protein is also up-regulated in tumor tissues, such as lung, prostate, melanoma, colorectal, breast cancer, and brain tumors, suggesting that it may play an important role in the development and progression of these diseases. Fibrinogen Alpha Chain (FGA) has been identified as an interactor of FGL1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGL1 and recombinant mouse FGA. Briefly, biotin-linked FGL1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGA-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human FGL1 and recombinant mouse FGA was shown in Figure 1, the EC50 for this effect is 0.07 ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| J Hepatol. | The role of Hepassocin in the development of non-alcoholic fatty liver disease Pubmed: 23792031 |
| Diabetes Management | Serum hepassocin concentrations in diabetic patients with or without nonalcoholic fatty liver disease Futuremedicine:Source |
| United States Patent Application | Compositions and Methods for Diagnosing Lung Cancer y2016:0077095.html |
| Cell | Obesity Drives STAT-1-Dependent NASH and STAT-3-Dependent HCC Doi: 10.1016/j.cell.2018.09.053 |
| Liver Research | Do different bariatric surgery procedures impact hepassocin plasma levels in patients with type 2 diabetes mellitus? |
| Impacts of Different Modes of Bariatric Surgery on Plasma Levels of Hepassocin in Patients with Diabetes Mellitus |