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Active Tensin 1 (TNS1)

TNS; MXRA6; Matrix-Remodelling Associated 6

ACTIVITY TEST

Tensin 1 (TNS1) is a protein localizes to focal adhesions, regions of the plasma membrane where the cell attaches to the extracellular matrix. It crosslinks actin filaments and contains a Src homology 2 (SH2) domain, which is often found in molecules involved in signal transduction. TNS1 contains binding domain, which allows it to interact with a variety of signaling molecules and transmembrane receptors, such as integrins and receptor tyrosine kinases. To test the effect of TNS1 protein on cell proliferation, A549 cells were seeded into triplicate wells of 96-well plates and allowed to attach, replaced with various concentrations of recombinant human TNS1. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 ℃. Cell viability was assessed by CCK-8 assay after incubation with recombinant human TNS1 for 72h. The result was shown in Figure 1. It was obvious that TNS1 significantly decreased cell viability of A549 cells. The ED50 of recombinant human TNS1 is 1.348 μg/ml.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPC802Hu01 Recombinant Tensin 1 (TNS1) Positive Control; Immunogen; SDS-PAGE; WB.
APC802Hu01 Active Tensin 1 (TNS1) Cell culture; Activity Assays.
PAC802Hu01 Polyclonal Antibody to Tensin 1 (TNS1) WB; IHC
LAC802Hu81 FITC-Linked Polyclonal Antibody to Tensin 1 (TNS1) WB; IHC; ICC; IF.
SEC802Hu ELISA Kit for Tensin 1 (TNS1) Enzyme-linked immunosorbent assay for Antigen Detection.