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Active Transmembrane Protease, Serine 2 (TMPRSS2)

PRSS10; Serine protease 10

ACTIVITY TEST

Transmembrane Protease, Serine 2 (TMPRSS2) is a type II transmembrane serine protease widely expressed in epithelial tissues, especially in the respiratory and gastrointestinal tracts. It plays critical roles in physiological processes like proprotein activation and tissue remodeling. Importantly, TMPRSS2 mediates viral entry by cleaving viral spike proteins, making it a key host factor in viral infections. TMPRSS2 binds to ACE2, facilitating the activation and entry of certain viruses into host cells.Thus a functional ELISA assay was conducted to detect the interaction of recombinant human TMPRSS2 and recombinant human ACE2. Briefly, TMPRSS2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACE2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TMPRSS2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human TMPRSS2 and recombinant human ACE2 was shown in Figure 1, the EC50 for this effect is 2.14076µg/mL.

USAGE

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
the journal of the international association for the study of pain TMPRSS2, a novel membrane-anchored mediator in cancer pain PubMed: 25734995
ournal of Anatomy ACE2 and TMPRSS2 in human saliva can adsorb to the oral mucosal epithelium 34590312
J Korean Med Sci Soluble ACE2 and TMPRSS2 Levels in the Serum of Asthmatic Patients Pubmed:35226423
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