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Active Insulin Like Growth Factor Binding Protein 5 (IGFBP5)
IBP5
- Product No.APC659Ra61
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 80%
- Isoelectric Point8.2
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
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ACTIVITY TEST
Insulin Like Growth Factor Binding Protein 5 (IGFBP5) is a protein that plays an important role in regulating the activity of Insulin Like Growth Factors (IGFs). It has a high affinity for IGFs and is widely distributed in various tissues. IGFBP5 can bind to Insulin Like Growth Factor 2 (IGF2) in the extracellular space. By binding to IGF2, it can modulate the bioavailability and half-life of IGF2, either enhancing or inhibiting the interaction of IGF2 with its receptors, thus influencing cell growth, proliferation, and differentiation.Thus a functional ELISA assay was conducted to detect the interaction of recombinant rat IGFBP5 and recombinant rabit IGF2. Briefly, IGFBP5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IGF2-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IGFBP5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant rat IGFBP5 and recombinant rabit IGF2 was shown in Figure 1, the EC50 for this effect is 7.021ug/mL.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Animal | Comparative expression profiling of insulin-like growth factor binding protein-5 in milk of Bos indicus and Bubalus bubalis during lactation Pubmed:25491373 |